Re: Digest for cell-culture-forum@googlegroups.com - 2 Messages in 2 Topics

[Suman S]

Horizontal hoods blow air on to you - on your face, which can be
deadly. You use mainly cancerous cells for your studies. The equipment
used for this purpose is ideally called a "bio-safety cabinet", and
not a hood. it looks like a hood but there's a lot of difference. in a
biosafety cabinet all air inside is sucked in; nothing is thrown at
you. there are lot of issues involved; you get a lot of stuff in the
internet. But the bottom line is - you should use a horizontal laminar
hood for this purpose

On Thu, Aug 1, 2013 at 12:15 PM, <cell-cult...@googlegroups.com> wrote:
> Today's Topic Summary
>
> Group: http://groups.google.com/group/cell-culture-forum/topics
>
> Cell culture hood [1 Update]
> Freeze collagen aliquots [1 Update]
>
> Cell culture hood
>
> VM <maniven...@gmail.com> Jul 29 10:04AM -0700
>
> Hello,
>
> I am wondering whether laminar flow hoods with just a horizontal air flow
> can be used as a cell culture hood? Even though i am not going to use any
> harmful chemicals or infectious agents in there, i dont want to introduce
> contamination to the culture. Do anybody have any experience or faced this
> kind of issue?
>
> Thanks,
> VM
>
>
>
> Freeze collagen aliquots
>
> Martin <martin....@gmail.com> Jul 29 08:48AM -0700
>
> Hi,
>
> I'd like to regularly coat some dishes with collagen (Rat Tail, Sigma
> C7661) for use with PC12 cells. As I only need a small amount each time, I
> would like to know about any experience in freezing collagen aliquots to
> about -20C. The vendors all state that it is not recommended, but the
> sources have some conflict in interest... Did you find any deterioration in
> quality? What is your thawing protocol? In what solution do you freeze it
> and does the concentration matter?
>
> Alternatively, how could I store an opened bottle of lyophilised collagen
> in sterile conditions?
>
> Thanks!
>
>
>
> --
> You received this message because you are subscribed to the Google Groups
> "Cell Culture Forum" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to cell-culture-fo...@googlegroups.com.
> For more options, visit https://groups.google.com/groups/opt_out.
>
>



--
Mr. Suman S
Asst. Professor
Karnataka College of Pharmacy
Bangalore