weird green threads on my slide

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aana bock

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Jun 26, 2014, 4:18:00 PM6/26/14
to cell-cult...@googlegroups.com, Anagha Sawant
Hi all
I have been trying to image my GFP transfection slide-HEK293 CELLS are used for transfection. I used methanol:acetone (1:1), DAPI and Mowiol with in between PBST washes. My tranfection works decent but i get these weird green thread like structures which illuminate in DAPI filter but not in GFP filter on microscope. Has anyone see these before?? I am attaching document with image. 
thanks for the help!!

aana 
cell transfection.docx
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