Hi,
Thanks for the reply and fixing the bug.
I originally import a 16-bit 3D stack which is subsequently converted into 8-bit, and the scale is set. A threshold is then applied to the image and converted into stack binary.
I have then populated the ROI manager with 14 points which should fit a sphere (they were define using one orthogonal plane but with points on different slices). When I try to fit a sphere to the points there is an error saying there are no usage points in the ROI manager (as you guessed). I am guessing I am defining the points in an incorrect way.
Thanks again,
Pete