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How to remove the gasket from glass chamber slide ?

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Pascal Hua

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May 12, 1998, 3:00:00 AM5/12/98
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Hi,

I am trying to remove the gasket from glass chamber slides, and I
am having problems. It leave enough residue that when I put the coverslip
on top, it does not work well. I amd using Lab-Teck 8 chambers, Nunc. The
advice from the tech guy there was to cut the gasket all the way through
at one corner to lift it clean. Well, it has not work extremly well yet.
Let me know if you have any sugestions,

Thanks,

Pascal Hua

ph...@bcm.tmc.edu

Pascal Hua

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May 12, 1998, 3:00:00 AM5/12/98
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MiiCarter

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May 12, 1998, 3:00:00 AM5/12/98
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We use the cover slip versions of Nunc LabTec incubation chambers instead of
the slides. The gaskets on these seem to peel off without difficulty,
allowing us to seal the whole base to a regular slide with Permafluor. It
feels like silicone rubber, and as you mention, starting at one corner is
easiest. Perhaps the extra flexibility of a cover glass makes removal easier,
or possibly they use a weaker bond to reduce the danger of breakage.

David Carter
Genomic Solutions
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Susana Zanello

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May 13, 1998, 3:00:00 AM5/13/98
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Hello!

The same has happened to me when using those glass chamber slides, with
the consequence of breaking the slide many times (especially when I was
loosing my patience!...). I have opted for using the plastic ones
(Permanox) instead, also from Lab-Tek, and they work great. You can easily
peel off the gasket. Of course, you have to need to make sure that your
cells grow and stick stick well on the plastic surface (which I generally
found it was not a problem).
Try them! I think you'll be satisfied.

Susana

At 10:41 AM 5/12/98 -0500, you wrote:
>Hi,


>
> I am trying to remove the gasket from glass chamber slides, and I
>am having problems. It leave enough residue that when I put the coverslip
>on top, it does not work well. I amd using Lab-Teck 8 chambers, Nunc. The
>advice from the tech guy there was to cut the gasket all the way through
>at one corner to lift it clean. Well, it has not work extremly well yet.
>Let me know if you have any sugestions,
>

> Thanks,
>
>Pascal Hua
>
>ph...@bcm.tmc.edu
>
>
Susana B. Zanello, PhD
Vitamin D, Skin and Bone Laboratory
Boston University School of Medicine
715 Albany St. M-1018
Boston, MA 02118
Fax: (617) 638 8882
Phone: (617) 638 8899

Norman Jay Kleiman

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May 18, 1998, 3:00:00 AM5/18/98
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have you tried the plastic version. the flexibility permits the gasket
to come off more smoothly and completely.

On Tue, 12 May 1998, Pascal Hua

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