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Two cents –
Check out https://pubmed.ncbi.nlm.nih.gov/21509745/
I frequently will use the Porod exponent as quantified in the program ScAtter and the flexibility plots described to characterize this property.
Also check out the work of Pau Bernado’s group characterizing polyubiquitin chains using SAXS and EOMs. Some great discussions of how flexibility manifests itself in SAS data and analysis at every stage.
Also see if your masses determined by Vc and I(0) greatly vary with those sensitive to protein volume and compactness like Vp and from the bead models. The latter calculations will be compromised by flexibility.
Kushol
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Richard
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