By default CHROMIXS displays the average intensity between 0.01 and 0.08 1/A. This is a range that tends to have relatively strong protein signal, and so this can be useful for reducing the overall noise in the intensity vs. frame number display. By default, RAW displays the total intensity across the entire q range of the dataset. This helps make sure that you don’t miss changes in the data due to things like accumulating radiation damage changing the low q values or a drift in the high q values due to beam motion, but leads to more noise in the intensity vs. frame number display. Basically, there’s tradeoffs either way.
If you want to display something essentially equivalent to CHROMIXS, right click on the series plot, go to the “Y Data (Left Axis)” sub menu, and select “Intensity in q range”. Then enter “0.01, 0.08” (without the quotes) and click OK. That will display something very similar to the CHROMIXS plot (RAW will be displaying total intensity and CHROMIXS will display average intensity, but overall it will look basically the same). See attached screenshots.
Let me know if that does the trick for you.
To Richard’s point about images, CHROMIXS only works with .dat files, so you don’t have to worry about that here.
All the best.
Jesse Hopkins, PhD
BioCAT, Sector 18
Advanced Photon Source