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PONE-D-22-06449Lifestyle risk factors and metabolic markers of cardiovascular diseases in Bangladeshi rural-to-urban male migrants compared with their non-migrant siblings: a sibling-pair comparative studyPLOS ONE

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Reviewer #1: The reviewed manuscript is based on original data, very actual and includes results of very interesting research topic. As indicates the title of the manuscript, the results of the manuscript are based only on the examination data of male sibling-pairs. Therefore I recommend indicate in the aim of the manuscript that only male sibling-pair data were analyzed and exclude from the Method section text and other parts of the manuscript, in which female sibling-pair selection, numbers of female sibling-pairs the exclusion criteria (for example, pregnant women) (line 153) and so on, were mentioned. You mention in the text of the manuscript term "CVDs risk factors": physical inactivity, smoking, overweight and obesity, abdominal obesity, low HDL. I did not find in the section Methods described how those factors were determined and classified. The only definition of hypertension and diabetes are presented (lines 329-330). So, please include the description of the methods of the examination and classification of the CVDs risk factors into the Methods section. Maybe you presented the mentioned description of the determination and classification of the CVDs risk factors in your previous publications: so, you could indicate such publication in the list of the references instead of including of additional paragraph into the section Methods of the manuscript.

Reviewer #2: This is an interesting work on male (in-country) rural-to-urban migrants on a major public health issue in Bangladesh, NCD risk factors. This study has shown how exposure of the people to an urban environment (physical and others) gradually develops acquire risk factors to develop CVD in future compared to their rural sibs. This is probably done for the first time in Bangladesh. The authors deserve special felicitation for this milestone piece.

5. Line 185: Reference has been made to the Supplementary file about the sampling technique. It could be mentioned briefly here also so that readers can understand it without reading the Supplementary file.

10. Table 1: Treatment history for diabetes and hypertension could be one of the determinants of their blood glucose and blood pressure levels. This has been mentioned by the authors in the Discussion section. Can we have the treatment history data here?

12. Table 4: It has been inadvertently labeled as Table 1. All ORs and their confidence intervals could be presented up to one decimal point. This will not lose any information, but the Table will appear clear and succinct. Please consider this for Table 2 also.

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1 This is an interesting work on male (in-country) rural-to-urban migrants on a major public health issue in Bangladesh, NCD risk factors. This study has shown how exposure of the people to an urban environment (physical and others) gradually develops acquire risk factors to develop CVD in future compared to their rural sibs. This is probably done for the first time in Bangladesh. The authors deserve special felicitation for this milestone piece. We thank the reviewer for appreciating our work and indeed it is the first of its kind in Bangladesh.

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