Isolation of protein

[vikas kumra]

Hi! U can try lowery's method for protein isolation, it can be
availble in any practical bk at ur clg library easily

On 12/12/09, shubhneet kaur <grewal.s...@gmail.com> wrote:
> hello frnds
> can anybody send me the protocol for isolation of proteins from rice grain
> ?its urgent. the method should be easy to use
>
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[mini here]

as far as my knowledge, lowry method is nt for isolation of protiens, it is for to check the presence of proteins. lyk u cn quantitate the protein present in the sample....... --- On Mon, 14/12/09, vikas kumra <kumra...@gmail.com> wrote:

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[r madhan]

hi first you tell me  from which sample are you going to isolate the protein

take care


with regards
madhan

[Chhavi Sharma]

Hi
Lowry method is used for estimation of protein

[nidhi gupta]

Mini is absolutely right........Lowery's method is for protein estimation not for its isolation.....
yes u can check the quantity of protein in given sample using spectrophotometer.

On Mon, Dec 14, 2009 at 10:55 PM, mini here <mini_h...@yahoo.co.in> wrote:

[senthil kumar]

Mini is absolutely right........Lowery's method is for protein estimation not for its isolation.....
yes u can check the quantity of protein in given sample using spectrophotometer.

 i accept all your thought.

how to know the protein quantity using spectrophotometer. please explain it. i have the doubt.

--
Ur's

S.Senthil Kumar M.Tech.,
Research Scholar
Department of Biotechnology
Anna University Tiruchirappalli
Tiruchirappalli - 620 024
Tamil Nadu
INDIA.

                                                                                   

[vikas kumra]

Ya its right lowry is for estimatiom, my mistake,
u can use phosphate buffer method to isolate protein, u cn found it in
"practical biochemistry" book...might it help u...

On 12/14/09, nidhi gupta <evergre...@gmail.com> wrote:
> Mini is absolutely right........Lowery's method is for protein estimation
> not for its isolation.....
> yes u can check the quantity of protein in given sample using
> spectrophotometer.
>
>
>
>
> On Mon, Dec 14, 2009 at 10:55 PM, mini here <mini_h...@yahoo.co.in>wrote:
>
>> as far as my knowledge, lowry method is nt for isolation of protiens, it
>> is
>> for to check the presence of proteins. lyk u cn quantitate the protein
>> present in the sample.......
>>

>> --- On *Mon, 14/12/09, vikas kumra <kumra...@gmail.com>* wrote:
>>
>>
>> From: vikas kumra <kumra...@gmail.com>
>> Subject: Isolation of protein
>> To: biote...@googlegroups.com
>> Date: Monday, 14 December, 2009, 10:28 PM
>>
>>
>> Hi! U can try lowery's method for protein isolation, it can be
>> availble in any practical bk at ur clg library easily
>>
>> On 12/12/09, shubhneet kaur

>> <grewal.s...@gmail.com<http://mc/compose?to=grewal.s...@gmail.com>>

>> wrote:
>> > hello frnds
>> > can anybody send me the protocol for isolation of proteins from rice
>> grain
>> > ?its urgent. the method should be easy to use
>> >
>> > --
>> > Are You Preparing for DBT JRF, NET JRF, GATE (XL)?
>> >
>> > * 5 Months Integrated Preparatory Course for IISc Entrance, NET JRF
>> > Entrance, GATE (XL) , DBT Entrance.
>> > * Discount: 5% off only for BIOTECNIKA members
>> > * Organizer: Gyan Bindu Academy, New Delhi
>> > * Batches Starting from: 9th July, 25th July, 10th August
>> > * Venue: Jia Sarai, New Delhi
>> >
>> > To sign up and claim your discount GoTo to
>> http://www.biotecnika.org/stores/
>> > _______________________________________________
>> > You received this message because you are subscribed to the Google
>> > Groups "BIOTECNIKA GLOBAL" group which is run by BIOTECNIKA
>> > ENCYCLOPEDIA.
>> > To post to this group send an email to

>> > biote...@googlegroups.com<http://mc/compose?to=biote...@googlegroups.com>

>>
>> --
>> Sent from my mobile device
>>
>> --
>> Are You Preparing for DBT JRF, NET JRF, GATE (XL)?
>>
>> * 5 Months Integrated Preparatory Course for IISc Entrance, NET JRF
>> Entrance, GATE (XL) , DBT Entrance.
>> * Discount: 5% off only for BIOTECNIKA members
>> * Organizer: Gyan Bindu Academy, New Delhi
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>>
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[senthil kumar]

thank you my friends...

[nidhi gupta]

this can be done by first plotting a standard OD graph and then plotting graph for different concentrations of the given sample.......
definitely we check the absorbence of sample at particular wavelength .......means use of spectrophotometer.

                                                                                   

--

[hradhaya bhatlawande]

Can you explain atp synthesis

[Mahender Singh]

do not worry just take .5 M tris solution you defenatly got result