[Virology] Calculating virus quantities and ratios

[jirasu]

I don´t know how to calculate a prosentage of viruses we managed to get from
our specimen,compared to the amount from the viruses that were put to the
specimen in the first place (% of the amount of viruses in the positive
RNA-control). And what were the amounts of viruses in PCR-units.
I´m quite desperate cause if I won´t get this done I can´t do the final
examn.
We put only 5ul of specimen in the DNA-strip when in the end the whole
amount of the separated RNA was 60ul.

Quantitation Report:

Positive RNA-control
Ct-value 24,88
Calc conc (copies) 8826
1 sample
Ct-value 28,99
Calc conc 527
2 sample
Ct-value 32,15
Calc conc 60

I´m grateful for any answere!
--
View this message in context: http://old.nabble.com/Calculating-virus-quantities-and-ratios-tp30648707p30648707.html
Sent from the Bio.net - Virology mailing list archive at Nabble.com.