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Use of Value of TCID50 result
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masud
May 16, 2012, 11:57:38 AM5/16/12
to bionet-...@moderators.isc.org
I have measured TCID50 value of virus: 1.6X10^4/ml. Now how do I use
the value in the real infection assay? If the infection fianl volume
is 100 microliter, for 1 TCID50 (of above value) or 100 TCID50 (of the
same virus stock) what volume we need to infect? Does the cell number
in 100 microliter is a concern or not? If any body can discuss.
Thanks.
the value in the real infection assay? If the infection fianl volume
is 100 microliter, for 1 TCID50 (of above value) or 100 TCID50 (of the
same virus stock) what volume we need to infect? Does the cell number
in 100 microliter is a concern or not? If any body can discuss.
Thanks.
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