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How to stain plant sections with toluidine blue
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Dr. M. Rehan Siddiqi
Mar 26, 2008, 7:32:33 AM3/26/08
to Plan...@magpie.bio.indiana.edu
In literature I have read that stem and roots section of angiosperms can be stained with toluidine blue stain. Can somebody tell me how to prepare this stain for staining and what is the procedure for staining sections with toluidine blue stain
Thanks
Thanks
Dr. M. Rehan Siddiqi,
Biology Department
Forman Christian College
Lahore, Pakistan
Take Care,
M. Rehan Siddiqi
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timb...@yahoo.com
Mar 27, 2008, 10:35:25 AM3/27/08
to bionet-plan...@magpie.bio.indiana.edu
There have been several discussions in this newsgroup about toluidine
blue. They can be found at the following link:
blue. They can be found at the following link:
A google search will provide many other sources, such as the
following:
BIOL2053: Hand sectioning and staining of sections
http://www.cavehill.uwi.edu/FPAS/bcs/courses/Biology/BIOL2053/2053proj/biol2053sect.htm
Creating Free Hand Sections of Plant Specimens
http://dbs.umt.edu/courses/biol316/freehand.htm
Lux A., Morita S., Abe J. and Ito K. 2005. An improved method for
clearing and staining free-hand sections and whole-mount samples. Ann
Bot (Lond). 96(6):989-96.
http://aob.oxfordjournals.org/cgi/content/full/96/6/989
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