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Control gRNA for direct gene knock out
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Christoph Seiler
Jan 9, 2018, 11:59:28 AM1/9/18
to bionet-organi...@moderators.isc.org
We are using Cripsr/Cas to directly knock out genes in zebrafish. We found this much more efficient and with less background than morpholinos. However, we need to design a good control. Ideally this binds in a dispensable part in the genome and induces 100% deletions without having an effect on the viability of the larvae? Any suggestions?
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