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Alkaline Phosphatase staining of endothelial cells
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chubbyjayu
Nov 22, 2009, 11:13:40 PM11/22/09
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Hi there!
My lab has been trying to target the alkaline phosphatase staining for
the subintestinal basket. The protocol we follow is from Zebrafish:
Nusslein-Volhard and Dahm.My problem is that although the basket gets
stained, there's a lot of overstaining on the yolk, which does not
allow for proper imaging. I have tried adjusting the amount of NBT/
BCIP I add, but to no avail.
Could anyone tell me why the overstaining occurs, and how to prevent
it?
Thanks.
My lab has been trying to target the alkaline phosphatase staining for
the subintestinal basket. The protocol we follow is from Zebrafish:
Nusslein-Volhard and Dahm.My problem is that although the basket gets
stained, there's a lot of overstaining on the yolk, which does not
allow for proper imaging. I have tried adjusting the amount of NBT/
BCIP I add, but to no avail.
Could anyone tell me why the overstaining occurs, and how to prevent
it?
Thanks.
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