Funduscopy zebrafish

[vireshdw...@gmail.com]

Good evening,
I'm an sub-researcher from Suriname South-America.
Working with the Anton de Kom University dep. Physiology.

We are doing research with the Zebrafish, mainly trying to see the retina of the zebrafish.
But i can't see the retina because there is a grey/black substance that is blocking the light of the microscope to go past that.

Are there any other methods for examining the retina of the Zebrafish?

[Jenny Lenkowski]

Hi Viresh,

That is the pigment of the retinal pigment epithelium. If you are examining larvae, you can use PTU (1-phenyl-2-thiourea) to prevent the melanin from developing and be able to see the retina that way. If you are doing histology on adult retinas, you will need to remore the retinal pigment epithelium to visualize the retina.

Jenny
Post-doctoral Fellow, Raymond Lab
University of Michigan

[htomas...@gmail.com]

In addition to the melanin in the retina older fish will have a shiny reflective layer made up of the iridiphores. We have a line of fish, sheer, which lacks these pigments. For younger fish you may be able to use albinos.