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Announcement of FIFTH (and FINAL) Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases
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Keith Joung
Feb 9, 2013, 7:28:20 PM2/9/13
to bionet-organi...@moderators.isc.org, jjo...@partners.org
Dear Colleagues,
We are writing again to solicit recommendations for appropriate genes
to target in our FIFTH (and FINAL) solicitation of zebrafish genes to
target with engineered nucleases. As in the previous (fourth) round,
we plan to construct 96 TALEN pairs. (Note that plasmids for the 96
pairs made for the fourth solicitation are nearly completed and will
be deposited with Addgene by the end of February.)
Each laboratory may submit up to TWO gene targets by completing an
Excel form that can be downloaded here: http://www.talengineering.org/download.htm.
Note that one gene recommendation should be placed on each of the
"Sheets" in the file. Place your first choice gene on the first sheet
and your second choice gene on the second sheet. Please note that ALL
required fields must be filled out - we will not accept or consider
any incomplete forms. For a gene to be considered appropriate for
this effort, we ask each recommender to verify on the form that
mutations in the gene of interest do not already exist and that
efforts to generate mutations in the recommended gene are not underway
elsewhere. VERY IMPORTANT NOTE: Each gene submitted MUST include its
Ensembl Gene ID (ENSDARG) the Ensembl Transcript ID (ENSDART), and the
gene name (e.g.-- Example: ENSDARG00000024771, ENSDART00000033574,
slc24a5). In the event that Ensembl has incorrectly annotated the
gene of interest, the full genomic sequence of your gene should be
included with exons denoted by upper case and introns in lower case.
Genes not correctly submitted in one of these formats will NOT be
considered for targeting.
Recommendations will be considered in the order in which they are
received. We will choose one gene for targeting from each lab until
we reach 96 gene targets. If we receive fewer than 96 targetable
"first choice" gene requests, we will then consider "second choice"
requests in the order in which we received the e-mails until we reach
96 gene targets.
Please e-mail your completed form to zebraf...@zincfingers.org at
or after (and not before) 12:00 Noon (Eastern US time) Friday,
February 22, 2013. Requests received before this date/time will not
be considered. Gene recommendations will only be accepted via e-
mail. Note that recommendations from scientists at commercial
entities will not be accepted.
With best regards,
Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital
We are writing again to solicit recommendations for appropriate genes
to target in our FIFTH (and FINAL) solicitation of zebrafish genes to
target with engineered nucleases. As in the previous (fourth) round,
we plan to construct 96 TALEN pairs. (Note that plasmids for the 96
pairs made for the fourth solicitation are nearly completed and will
be deposited with Addgene by the end of February.)
Each laboratory may submit up to TWO gene targets by completing an
Excel form that can be downloaded here: http://www.talengineering.org/download.htm.
Note that one gene recommendation should be placed on each of the
"Sheets" in the file. Place your first choice gene on the first sheet
and your second choice gene on the second sheet. Please note that ALL
required fields must be filled out - we will not accept or consider
any incomplete forms. For a gene to be considered appropriate for
this effort, we ask each recommender to verify on the form that
mutations in the gene of interest do not already exist and that
efforts to generate mutations in the recommended gene are not underway
elsewhere. VERY IMPORTANT NOTE: Each gene submitted MUST include its
Ensembl Gene ID (ENSDARG) the Ensembl Transcript ID (ENSDART), and the
gene name (e.g.-- Example: ENSDARG00000024771, ENSDART00000033574,
slc24a5). In the event that Ensembl has incorrectly annotated the
gene of interest, the full genomic sequence of your gene should be
included with exons denoted by upper case and introns in lower case.
Genes not correctly submitted in one of these formats will NOT be
considered for targeting.
Recommendations will be considered in the order in which they are
received. We will choose one gene for targeting from each lab until
we reach 96 gene targets. If we receive fewer than 96 targetable
"first choice" gene requests, we will then consider "second choice"
requests in the order in which we received the e-mails until we reach
96 gene targets.
Please e-mail your completed form to zebraf...@zincfingers.org at
or after (and not before) 12:00 Noon (Eastern US time) Friday,
February 22, 2013. Requests received before this date/time will not
be considered. Gene recommendations will only be accepted via e-
mail. Note that recommendations from scientists at commercial
entities will not be accepted.
With best regards,
Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital
Keith Joung
Feb 28, 2013, 9:51:21 PM2/28/13
to bionet-organi...@moderators.isc.org
Dear all,
We are pleased to announce the list of 96 zebrafish genes and target
sites for which we will make FLASH-assembled TALENs for the community
as part of our FIFTH (and FINAL) NIH-sponsored solicitation can be
found below. We hope to deposit the plasmids encoding these TALENs
with Addgene in the coming months. We will write with updates as we
make those deposits.
Best,
Keith Joung
Randall Peterson
Joanna Yeh
Jon Foley
Deepak Reyon
Jeff Sander
Massachusetts General Hospital
ID Gene Name Target Site
1 ZebrafishCommunity-abcg2a-left TGTCCTCATCCCCGTCCC
1 ZebrafishCommunity-abcg2a-right TTGATGTTGTGGAAGCTG
2 ZebrafishCommunity-apoeb-left TGCCAGGCTCGTAGCCTG
2 ZebrafishCommunity-apoeb-right TCCTCCCATCTGGGCTGA
3 ZebrafishCommunity-asun-left TAACTCAAGCCAACTCCA
3 ZebrafishCommunity-asun-right TGAACTCAATCTTCCTGC
4 ZebrafishCommunity-atf6-left TCCAACCAGCAAAAAGAC
4 ZebrafishCommunity-atf6-right TAGGCTGAATGGAAACCT
5 ZebrafishCommunity-CHCHD4-left TCTACCTAATGGCGACAT
5 ZebrafishCommunity-CHCHD4-right TGGCCATGCCGCCCAAAC
6 ZebrafishCommunity-col22A1-left TGGATGGCGAGAACAGGG
6 ZebrafishCommunity-col22A1-right TCCTTCGTCAGGCCCACA
7 ZebrafishCommunity-Cyp19a1-left TGCTCCAGCCCTGTGGAA
7 ZebrafishCommunity-Cyp19a1-right TCCACCACAGCCTCGCCG
8 ZebrafishCommunity-CYP20A1-left TAGACTTTCCTGAAAAGC
8 ZebrafishCommunity-CYP20A1-right TGTCACAGCAAAAATGGC
9 ZebrafishCommunity-dcps-left TGAACAGTCCACCGATAA
9 ZebrafishCommunity-dcps-right TAGAATGCGGGTGAGCTG
10 ZebrafishCommunity-ddx26b-left TGAAGGCCAGCACGACAC
10 ZebrafishCommunity-ddx26b-right TTGTAAGGCATGACGAAG
11 ZebrafishCommunity-def6a-left TCAGAACTGCTCAAGTCC
11 ZebrafishCommunity-def6a-right TCTCCACATCCAGAGCTG
12 ZebrafishCommunity-DLK1(different_cut)-left TGAGGTACAGGCAGCTGG
12 ZebrafishCommunity-DLK1(different_cut)-right TGGCCTGGTCCTGGATCA
13 ZebrafishCommunity-dyrk1aa-left TGTTCCGAAACCACCTGT
13 ZebrafishCommunity-dyrk1aa-right TACAGGTTATAGGACAGC
14 ZebrafishCommunity-dyrk1b-left TCCTGAACCAGGCCCAGA
14 ZebrafishCommunity-dyrk1b-right TGTTTGTTCATGAGCTCA
15 ZebrafishCommunity-eif2a-left TCACCTACAGAAAGTGTC
15 ZebrafishCommunity-eif2a-right TGCTCGGCTGTGATCCAG
16 ZebrafishCommunity-eomesb-left TCTTCTCACCCGGAGGCG
16 ZebrafishCommunity-eomesb-right TCACCACAAACTACCGGA
17 ZebrafishCommunity-epd-left TGGGCTTCTCATCACTCT
17 ZebrafishCommunity-epd-right TGGTCAGCTGTGGTGAAT
18 ZebrafishCommunity-f11r-left TTGACTTGCAATGTTCCT
18 ZebrafishCommunity-f11r-right TCTACTCTGGGTGTTGCT
19 ZebrafishCommunity-fabp3-left TGTTTGTCTTCTGTTGTT
19 ZebrafishCommunity-fabp3-right TCCCTCCATCTAAAGTTA
20 ZebrafishCommunity-fastkd3-left TACCTGTCTTCTAATGAG
20 ZebrafishCommunity-fastkd3-right TACAAGAGGAGAGGCGTT
21 ZebrafishCommunity-FoxF2a-left TCAGGTCCAGCCCGGCAT
21 ZebrafishCommunity-FoxF2a-right TGCGTGTTTTGGAGAGCC
22 ZebrafishCommunity-fshr-left TGTTGTCAATGATGCTGT
22 ZebrafishCommunity-fshr-right TCGGTGTTTGCCATGGAG
23 ZebrafishCommunity-GRIN2B-left TCCAGGTGTACCCGTACC
23 ZebrafishCommunity-GRIN2B-right TCATCTTCCAGGTGGCGC
24 ZebrafishCommunity-GRM2b-left TATCTTGCCCGGCTTGAA
24 ZebrafishCommunity-GRM2b-right TTTCGAGCAGGTGTCCAG
25 ZebrafishCommunity-hcrt-left TACAAGACAGGGGACAGA
25 ZebrafishCommunity-hcrt-right TCTTACCTTAGCTGTG
26 ZebrafishCommunity-hs3st3l-left TCACCTGCATCTCCATCC
26 ZebrafishCommunity-hs3st3l-right TCGGAGGAGTCGCAGCAT
27 ZebrafishCommunity-hsd17b1-left TTGTTCTCATCACTGGAT
27 ZebrafishCommunity-hsd17b1-right TGCACAGCAAGGCTGAGT
28 ZebrafishCommunity-Hsf1-left TCACCGGGAACAATGTGC
28 ZebrafishCommunity-Hsf1-right TCCACCAGCGTCCAGAGT
29 ZebrafishCommunity-igf3-left TGCCAGACAACACTGAAG
29 ZebrafishCommunity-igf3-right TCCACTAGTTCTCGTCCG
30 ZebrafishCommunity-igfbp1a-left TGCTTGCCGAAACCGGGA
30 ZebrafishCommunity-igfbp1a-right TGTCCCCGGGTGAGCGCG
31 ZebrafishCommunity-igfbp1b-left TCCGGGATCCGGGCTGCG
31 ZebrafishCommunity-igfbp1b-right TCGCCGCTCCGCAGGGCG
32 ZebrafishCommunity-Impacthomolog(mouse)-left TGGACAAATTAGAGGATC
32 ZebrafishCommunity-Impacthomolog(mouse)-right TCCTCTATCTGAGACTGC
33 ZebrafishCommunity-INF2-left TAAACGCCCTGATCTTCA
33 ZebrafishCommunity-INF2-right TGCGCATCTTGTCTCTCT
34 ZebrafishCommunity-inhibitorofDNAbinding3-left TCAACATTGGATATCTAT
34 ZebrafishCommunity-inhibitorofDNAbinding3-right TCTGGACACTGGGATAAA
35 ZebrafishCommunity-kcnma1a-left TGCCTGGCACAGGGCCTG
35 ZebrafishCommunity-kcnma1a-right TGACCTCATGGAGAAGAG
36 ZebrafishCommunity-kcnma1b-left TGCCTGGCACAGGGGCT
36 ZebrafishCommunity-kcnma1b-right TCATTGAGAACAGGTT
37 ZebrafishCommunity-KISS1RA-left TTTTCTGGTGAAGACTGG
37 ZebrafishCommunity-KISS1RA-right TTCTGAAAGAGCCATTCA
38 ZebrafishCommunity-KISS1RB-left TGATGGCAGAAACTAACA
38 ZebrafishCommunity-KISS1RB-right TTGCACATGATGTGCTCT
39 ZebrafishCommunity-klf8-left TGAGCCCACAGACCTCAA
39 ZebrafishCommunity-klf8-right TGATGATGCAGAGCGGTG
40 ZebrafishCommunity-klf4a-left TGTCTCACCTGAAACGCC
40 ZebrafishCommunity-klf4a-right TGGTCAGCGCAGGTCGCA
41 ZebrafishCommunity-LBH-left TGTGCTCGTCTTCAGATC
41 ZebrafishCommunity-LBH-right TACAGCAGCGCTCGAAGT
42 ZebrafishCommunity-lycat-left TCCTTCATCTTCATCCAG
42 ZebrafishCommunity-lycat-right TGCTCATGTGTGTGCGGT
43 ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-left
TGATCTGTTCCTGGTAGC
43 ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-
right TTCCAGTCCAAGGGCATG
44 ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-
left TGTTCCTGGTGGCGGTGC
44 ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-
right TGAGTGCTCCAAACCAAG
45 ZebrafishCommunity-mef2d-left TGGGGAGGAAAAAGATCC
45 ZebrafishCommunity-mef2d-right TGTCTGTTACGCTCGTCT
46 ZebrafishCommunity-meis2b-left TGCTGGCGCTGGTGTTTG
46 ZebrafishCommunity-meis2b-right TCTCTCGGTGTGCATGTG
47 ZebrafishCommunity-mical2b-left TGGGGGAGACGGAGGAAG
47 ZebrafishCommunity-mical2b-right TTCTCAAAAAGCTGACCG
48 ZebrafishCommunity-mtdhb-left TGCCAGCACAATAGATTG
48 ZebrafishCommunity-mtdhb-right TGCGACGCGATCTCTTGC
49 ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-left
TGCCGCCAACTGGCTGAA
49 ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-right
TTTTGCGCTTGTTCTG
50 ZebrafishCommunity-Neurl1a-left TCTCTAGGTGGACCATTT
50 ZebrafishCommunity-Neurl1a-right TGGGCTTGTGATGGCATC
51 ZebrafishCommunity-NPHP5/iqcb1-left TGGTCTGGCTGCTTTCAG
51 ZebrafishCommunity-NPHP5/iqcb1-right TGGCAGAAATTCATGACT
52 ZebrafishCommunity-NR1I2-left TCGGAGCTCGCCGTGTCT
52 ZebrafishCommunity-NR1I2-right TAAGCGGGACATTGACGT
53 ZebrafishCommunity-pax1a-left TGAAAAACATACGGGACT
53 ZebrafishCommunity-pax1a-right TCCCATGCAAAAATTCCC
54 ZebrafishCommunity-pax1b-left TGGGAATCAGACCCTGCG
54 ZebrafishCommunity-pax1b-right TCCATGAGAGACCCTG
55 ZebrafishCommunity-pax7a-left TTAGAAGTCTCAACACCT
55 ZebrafishCommunity-pax7a-right TCCACCGAGTTGATTGA
56 ZebrafishCommunity-pbx2-left TGGAGCAGACCCCGCGGG
56 ZebrafishCommunity-pbx2-right TGCGGTTGTCGTGTCCGT
57 ZebrafishCommunity-pcdh10a-left TCGCCGAAGATCTGGGGC
57 ZebrafishCommunity-pcdh10a-right TGGAAACGGCGCGCGGAA
58 ZebrafishCommunity-pls1-left TACGGGAGATTGTAGAGA
58 ZebrafishCommunity-pls1-right TTCTCATCTTTGTTGGTG
59 ZebrafishCommunity-Prdx3-left TGCTTTCACGCATTGCAC
59 ZebrafishCommunity-Prdx3-right TCTCCCGATGGTGGCTGC
60 ZebrafishCommunity-prp19-left TTCCAGTTTCAAACGAAG
60 ZebrafishCommunity-prp19-right TTGGAAACAGGGGACACA
61 ZebrafishCommunity-prrx1a-left TGGGTTTCGTCAATTTGC
61 ZebrafishCommunity-prrx1a-right TGTCCATTACATGAGCAT
62 ZebrafishCommunity-rbfox2-left TGAGGAGGGCGCAGTGGT
62 ZebrafishCommunity-rbfox2-right TGGAGGAGGAGGAGGAGG
63 ZebrafishCommunity-rbp4-left TGTTAAGGCTCTGTATAG
63 ZebrafishCommunity-rbp4-right TCCTGAGCCCAGCAAGTG
64 ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive) -left
TCTTTTTGTGCTGCGCCA
64 ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive) -right
TTCATGCCTGTAGAAGAC
65 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorb-left
TGGAGAAACAGAAGATGA
65 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorb-right
TCCAAAAGTGAAGACAGC
66 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-left
TAAGAGGGCGGAGCTTTT
66 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-right
TCTAAGTAACTCAGTGGG
67 ZebrafishCommunity-sdc4-left TGGAGTCCTCCGGCAACT
67 ZebrafishCommunity-sdc4-right TTTAAGTCGGTGAACTCA
68 ZebrafishCommunity-sdcbp-left TCTCCCCGAACCTTCCAG
68 ZebrafishCommunity-sdcbp-right TGAATCATAGTAGGTACT
69 ZebrafishCommunity-sdccag8-left TGGTTCCCATCATAAACA
69 ZebrafishCommunity-sdccag8-right TCAGCCTCCAGGTGCTGA
70 ZebrafishCommunity-si:dkey-208k22.3-left TTATTCTCAGGCCAACTT
70 ZebrafishCommunity-si:dkey-208k22.3-right TTTCGACTTGTGCATCTT
71 ZebrafishCommunity-six4a-left TTTGTTTGCGCACAGTTC
71 ZebrafishCommunity-six4a-right TGAGAAAGCCAAAGAGGT
72 ZebrafishCommunity-slc17a9a-left TCTCCATTGGTCACGGAT
72 ZebrafishCommunity-slc17a9a-right TCTAGCCATGGTGACGGC
73 ZebrafishCommunity-slc17a9b-left TGCTCTACTGTGCGCGTG
73 ZebrafishCommunity-slc17a9b-right TCCGCCATGCTCACGGCA
74 ZebrafishCommunity-slit3-left TGGACAGGAATAACATCA
74 ZebrafishCommunity-slit3-right TTGAGGCCAGAGAAGTCC
75 ZebrafishCommunity-slmapb-left TGGAGGCCAGTGAGTCTG
75 ZebrafishCommunity-slmapb-right TGCAGTTCCTGGTTCAGC
76 ZebrafishCommunity-smyd1a-left TGGAGGTGTTCGGGGCCG
76 ZebrafishCommunity-smyd1a-right TCTCTGGTACCCCGCAGA
77 ZebrafishCommunity-snai1b-left TGAAGTTTCGAGGGGATA
77 ZebrafishCommunity-snai1b-right TCTTGACAAGAAATGAGC
78 ZebrafishCommunity-spermassociatedantigen7-left TTCTGGGCTCGATCTTGA
78 ZebrafishCommunity-spermassociatedantigen7-right TGATCGTGAACGGTTGGA
79 ZebrafishCommunity-sqstm1-left TGTCGATGACAGTGAAAG
79 ZebrafishCommunity-sqstm1-right TCCTTGTTGCAGTCCTCC
80 ZebrafishCommunity-srf-left TGGACGCTCTGGTAATGG
80 ZebrafishCommunity-srf-right TGTGCTGCCCACCTGTAG
81 ZebrafishCommunity-Stat3-left TGCAGCAGTTGGAGACGC
81 ZebrafishCommunity-Stat3-right TCGCTGTACAGGTGATAC
82 ZebrafishCommunity-stxbp1a-left TCAGCTTGCCCTGACCCC
82 ZebrafishCommunity-stxbp1a-right TGGAGGCACGGCTCTTCA
83 ZebrafishCommunity-Sun1-left TGGATTTTTCCCGTCTGC
83 ZebrafishCommunity-Sun1-right TTATCCGGAGTACAGTGA
84 ZebrafishCommunity-swap70b-left TAAGGGACGAGCTTCTC
84 ZebrafishCommunity-swap70b-right TCCAGGGCTGTGAAGG
85 ZebrafishCommunity-SYNE2-left TATGGGATGAGCTGGACC
85 ZebrafishCommunity-SYNE2-right TCGCCCTCTAACTCAGCT
86 ZebrafishCommunity-tbx4-left TGCTTCAGGAGAAGGCCT
86 ZebrafishCommunity-tbx4-right TGGGCAACGGTCATTCCT
87 ZebrafishCommunity-tetmethylcytosinedioxygenase1(previous-left
TCAACTCGCGCATCCACA
87 ZebrafishCommunity-tetmethylcytosinedioxygenase1(previous-right
TCATGGAAGCCTTCACCC
88 ZebrafishCommunity-tetmethylcytosinedioxygenase3-left
TTATTAGGCACTTTTTTG
88 ZebrafishCommunity-tetmethylcytosinedioxygenase3-right
TGTGGACAGGAAAGTGCA
89 ZebrafishCommunity-
tetmethylcytosinedioxygenase3(previoussi:ch211-220a11.1)-left
TCAAATCCTGAATCTCAA
89 ZebrafishCommunity-
tetmethylcytosinedioxygenase3(previoussi:ch211-220a11.1)-right
TTGTGCTGGCATTTTGAC
90 ZebrafishCommunity-TIMM22-left TTGTTCTGGGAGGTGCT
90 ZebrafishCommunity-TIMM22-right TTTGCATCAATGCCGG
91 ZebrafishCommunity-tppp3-left TGAGGGTTCGGGTGAAAT
91 ZebrafishCommunity-tppp3-right TTTACGAAATGCCATCTC
92 ZebrafishCommunity-trappc11-left TCCTATGAGTGGTACATT
92 ZebrafishCommunity-trappc11-right TGTTCATCCAGCCCGTCT
93 ZebrafishCommunity-ucma-left TGCTGATCGCGCTGACTG
93 ZebrafishCommunity-ucma-right TTTTCCATCTTTAACA
94 ZebrafishCommunity-vclbAL772263.1-left TGGCCCAGCAGATCTCCC
94 ZebrafishCommunity-vclbAL772263.1-right TCCACCTCTCCCTCCTCG
95 ZebrafishCommunity-ybx1-left TGAGCAGCGAGGCCGAGA
95 ZebrafishCommunity-ybx1-right TCCGCGTCGGCGGCAGGC
96 ZebrafishCommunity-yjefn3-left TTGAGACGGAGCTCCTGA
96 ZebrafishCommunity-yjefn3-right TCAATCAGCTGCTGCTGG
We are pleased to announce the list of 96 zebrafish genes and target
sites for which we will make FLASH-assembled TALENs for the community
as part of our FIFTH (and FINAL) NIH-sponsored solicitation can be
found below. We hope to deposit the plasmids encoding these TALENs
with Addgene in the coming months. We will write with updates as we
make those deposits.
Best,
Keith Joung
Randall Peterson
Joanna Yeh
Deepak Reyon
Jeff Sander
Massachusetts General Hospital
ID Gene Name Target Site
1 ZebrafishCommunity-abcg2a-left TGTCCTCATCCCCGTCCC
1 ZebrafishCommunity-abcg2a-right TTGATGTTGTGGAAGCTG
2 ZebrafishCommunity-apoeb-left TGCCAGGCTCGTAGCCTG
2 ZebrafishCommunity-apoeb-right TCCTCCCATCTGGGCTGA
3 ZebrafishCommunity-asun-left TAACTCAAGCCAACTCCA
3 ZebrafishCommunity-asun-right TGAACTCAATCTTCCTGC
4 ZebrafishCommunity-atf6-left TCCAACCAGCAAAAAGAC
4 ZebrafishCommunity-atf6-right TAGGCTGAATGGAAACCT
5 ZebrafishCommunity-CHCHD4-left TCTACCTAATGGCGACAT
5 ZebrafishCommunity-CHCHD4-right TGGCCATGCCGCCCAAAC
6 ZebrafishCommunity-col22A1-left TGGATGGCGAGAACAGGG
6 ZebrafishCommunity-col22A1-right TCCTTCGTCAGGCCCACA
7 ZebrafishCommunity-Cyp19a1-left TGCTCCAGCCCTGTGGAA
7 ZebrafishCommunity-Cyp19a1-right TCCACCACAGCCTCGCCG
8 ZebrafishCommunity-CYP20A1-left TAGACTTTCCTGAAAAGC
8 ZebrafishCommunity-CYP20A1-right TGTCACAGCAAAAATGGC
9 ZebrafishCommunity-dcps-left TGAACAGTCCACCGATAA
9 ZebrafishCommunity-dcps-right TAGAATGCGGGTGAGCTG
10 ZebrafishCommunity-ddx26b-left TGAAGGCCAGCACGACAC
10 ZebrafishCommunity-ddx26b-right TTGTAAGGCATGACGAAG
11 ZebrafishCommunity-def6a-left TCAGAACTGCTCAAGTCC
11 ZebrafishCommunity-def6a-right TCTCCACATCCAGAGCTG
12 ZebrafishCommunity-DLK1(different_cut)-left TGAGGTACAGGCAGCTGG
12 ZebrafishCommunity-DLK1(different_cut)-right TGGCCTGGTCCTGGATCA
13 ZebrafishCommunity-dyrk1aa-left TGTTCCGAAACCACCTGT
13 ZebrafishCommunity-dyrk1aa-right TACAGGTTATAGGACAGC
14 ZebrafishCommunity-dyrk1b-left TCCTGAACCAGGCCCAGA
14 ZebrafishCommunity-dyrk1b-right TGTTTGTTCATGAGCTCA
15 ZebrafishCommunity-eif2a-left TCACCTACAGAAAGTGTC
15 ZebrafishCommunity-eif2a-right TGCTCGGCTGTGATCCAG
16 ZebrafishCommunity-eomesb-left TCTTCTCACCCGGAGGCG
16 ZebrafishCommunity-eomesb-right TCACCACAAACTACCGGA
17 ZebrafishCommunity-epd-left TGGGCTTCTCATCACTCT
17 ZebrafishCommunity-epd-right TGGTCAGCTGTGGTGAAT
18 ZebrafishCommunity-f11r-left TTGACTTGCAATGTTCCT
18 ZebrafishCommunity-f11r-right TCTACTCTGGGTGTTGCT
19 ZebrafishCommunity-fabp3-left TGTTTGTCTTCTGTTGTT
19 ZebrafishCommunity-fabp3-right TCCCTCCATCTAAAGTTA
20 ZebrafishCommunity-fastkd3-left TACCTGTCTTCTAATGAG
20 ZebrafishCommunity-fastkd3-right TACAAGAGGAGAGGCGTT
21 ZebrafishCommunity-FoxF2a-left TCAGGTCCAGCCCGGCAT
21 ZebrafishCommunity-FoxF2a-right TGCGTGTTTTGGAGAGCC
22 ZebrafishCommunity-fshr-left TGTTGTCAATGATGCTGT
22 ZebrafishCommunity-fshr-right TCGGTGTTTGCCATGGAG
23 ZebrafishCommunity-GRIN2B-left TCCAGGTGTACCCGTACC
23 ZebrafishCommunity-GRIN2B-right TCATCTTCCAGGTGGCGC
24 ZebrafishCommunity-GRM2b-left TATCTTGCCCGGCTTGAA
24 ZebrafishCommunity-GRM2b-right TTTCGAGCAGGTGTCCAG
25 ZebrafishCommunity-hcrt-left TACAAGACAGGGGACAGA
25 ZebrafishCommunity-hcrt-right TCTTACCTTAGCTGTG
26 ZebrafishCommunity-hs3st3l-left TCACCTGCATCTCCATCC
26 ZebrafishCommunity-hs3st3l-right TCGGAGGAGTCGCAGCAT
27 ZebrafishCommunity-hsd17b1-left TTGTTCTCATCACTGGAT
27 ZebrafishCommunity-hsd17b1-right TGCACAGCAAGGCTGAGT
28 ZebrafishCommunity-Hsf1-left TCACCGGGAACAATGTGC
28 ZebrafishCommunity-Hsf1-right TCCACCAGCGTCCAGAGT
29 ZebrafishCommunity-igf3-left TGCCAGACAACACTGAAG
29 ZebrafishCommunity-igf3-right TCCACTAGTTCTCGTCCG
30 ZebrafishCommunity-igfbp1a-left TGCTTGCCGAAACCGGGA
30 ZebrafishCommunity-igfbp1a-right TGTCCCCGGGTGAGCGCG
31 ZebrafishCommunity-igfbp1b-left TCCGGGATCCGGGCTGCG
31 ZebrafishCommunity-igfbp1b-right TCGCCGCTCCGCAGGGCG
32 ZebrafishCommunity-Impacthomolog(mouse)-left TGGACAAATTAGAGGATC
32 ZebrafishCommunity-Impacthomolog(mouse)-right TCCTCTATCTGAGACTGC
33 ZebrafishCommunity-INF2-left TAAACGCCCTGATCTTCA
33 ZebrafishCommunity-INF2-right TGCGCATCTTGTCTCTCT
34 ZebrafishCommunity-inhibitorofDNAbinding3-left TCAACATTGGATATCTAT
34 ZebrafishCommunity-inhibitorofDNAbinding3-right TCTGGACACTGGGATAAA
35 ZebrafishCommunity-kcnma1a-left TGCCTGGCACAGGGCCTG
35 ZebrafishCommunity-kcnma1a-right TGACCTCATGGAGAAGAG
36 ZebrafishCommunity-kcnma1b-left TGCCTGGCACAGGGGCT
36 ZebrafishCommunity-kcnma1b-right TCATTGAGAACAGGTT
37 ZebrafishCommunity-KISS1RA-left TTTTCTGGTGAAGACTGG
37 ZebrafishCommunity-KISS1RA-right TTCTGAAAGAGCCATTCA
38 ZebrafishCommunity-KISS1RB-left TGATGGCAGAAACTAACA
38 ZebrafishCommunity-KISS1RB-right TTGCACATGATGTGCTCT
39 ZebrafishCommunity-klf8-left TGAGCCCACAGACCTCAA
39 ZebrafishCommunity-klf8-right TGATGATGCAGAGCGGTG
40 ZebrafishCommunity-klf4a-left TGTCTCACCTGAAACGCC
40 ZebrafishCommunity-klf4a-right TGGTCAGCGCAGGTCGCA
41 ZebrafishCommunity-LBH-left TGTGCTCGTCTTCAGATC
41 ZebrafishCommunity-LBH-right TACAGCAGCGCTCGAAGT
42 ZebrafishCommunity-lycat-left TCCTTCATCTTCATCCAG
42 ZebrafishCommunity-lycat-right TGCTCATGTGTGTGCGGT
43 ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-left
TGATCTGTTCCTGGTAGC
43 ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-
right TTCCAGTCCAAGGGCATG
44 ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-
left TGTTCCTGGTGGCGGTGC
44 ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-
right TGAGTGCTCCAAACCAAG
45 ZebrafishCommunity-mef2d-left TGGGGAGGAAAAAGATCC
45 ZebrafishCommunity-mef2d-right TGTCTGTTACGCTCGTCT
46 ZebrafishCommunity-meis2b-left TGCTGGCGCTGGTGTTTG
46 ZebrafishCommunity-meis2b-right TCTCTCGGTGTGCATGTG
47 ZebrafishCommunity-mical2b-left TGGGGGAGACGGAGGAAG
47 ZebrafishCommunity-mical2b-right TTCTCAAAAAGCTGACCG
48 ZebrafishCommunity-mtdhb-left TGCCAGCACAATAGATTG
48 ZebrafishCommunity-mtdhb-right TGCGACGCGATCTCTTGC
49 ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-left
TGCCGCCAACTGGCTGAA
49 ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-right
TTTTGCGCTTGTTCTG
50 ZebrafishCommunity-Neurl1a-left TCTCTAGGTGGACCATTT
50 ZebrafishCommunity-Neurl1a-right TGGGCTTGTGATGGCATC
51 ZebrafishCommunity-NPHP5/iqcb1-left TGGTCTGGCTGCTTTCAG
51 ZebrafishCommunity-NPHP5/iqcb1-right TGGCAGAAATTCATGACT
52 ZebrafishCommunity-NR1I2-left TCGGAGCTCGCCGTGTCT
52 ZebrafishCommunity-NR1I2-right TAAGCGGGACATTGACGT
53 ZebrafishCommunity-pax1a-left TGAAAAACATACGGGACT
53 ZebrafishCommunity-pax1a-right TCCCATGCAAAAATTCCC
54 ZebrafishCommunity-pax1b-left TGGGAATCAGACCCTGCG
54 ZebrafishCommunity-pax1b-right TCCATGAGAGACCCTG
55 ZebrafishCommunity-pax7a-left TTAGAAGTCTCAACACCT
55 ZebrafishCommunity-pax7a-right TCCACCGAGTTGATTGA
56 ZebrafishCommunity-pbx2-left TGGAGCAGACCCCGCGGG
56 ZebrafishCommunity-pbx2-right TGCGGTTGTCGTGTCCGT
57 ZebrafishCommunity-pcdh10a-left TCGCCGAAGATCTGGGGC
57 ZebrafishCommunity-pcdh10a-right TGGAAACGGCGCGCGGAA
58 ZebrafishCommunity-pls1-left TACGGGAGATTGTAGAGA
58 ZebrafishCommunity-pls1-right TTCTCATCTTTGTTGGTG
59 ZebrafishCommunity-Prdx3-left TGCTTTCACGCATTGCAC
59 ZebrafishCommunity-Prdx3-right TCTCCCGATGGTGGCTGC
60 ZebrafishCommunity-prp19-left TTCCAGTTTCAAACGAAG
60 ZebrafishCommunity-prp19-right TTGGAAACAGGGGACACA
61 ZebrafishCommunity-prrx1a-left TGGGTTTCGTCAATTTGC
61 ZebrafishCommunity-prrx1a-right TGTCCATTACATGAGCAT
62 ZebrafishCommunity-rbfox2-left TGAGGAGGGCGCAGTGGT
62 ZebrafishCommunity-rbfox2-right TGGAGGAGGAGGAGGAGG
63 ZebrafishCommunity-rbp4-left TGTTAAGGCTCTGTATAG
63 ZebrafishCommunity-rbp4-right TCCTGAGCCCAGCAAGTG
64 ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive) -left
TCTTTTTGTGCTGCGCCA
64 ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive) -right
TTCATGCCTGTAGAAGAC
65 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorb-left
TGGAGAAACAGAAGATGA
65 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorb-right
TCCAAAAGTGAAGACAGC
66 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-left
TAAGAGGGCGGAGCTTTT
66 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-right
TCTAAGTAACTCAGTGGG
67 ZebrafishCommunity-sdc4-left TGGAGTCCTCCGGCAACT
67 ZebrafishCommunity-sdc4-right TTTAAGTCGGTGAACTCA
68 ZebrafishCommunity-sdcbp-left TCTCCCCGAACCTTCCAG
68 ZebrafishCommunity-sdcbp-right TGAATCATAGTAGGTACT
69 ZebrafishCommunity-sdccag8-left TGGTTCCCATCATAAACA
69 ZebrafishCommunity-sdccag8-right TCAGCCTCCAGGTGCTGA
70 ZebrafishCommunity-si:dkey-208k22.3-left TTATTCTCAGGCCAACTT
70 ZebrafishCommunity-si:dkey-208k22.3-right TTTCGACTTGTGCATCTT
71 ZebrafishCommunity-six4a-left TTTGTTTGCGCACAGTTC
71 ZebrafishCommunity-six4a-right TGAGAAAGCCAAAGAGGT
72 ZebrafishCommunity-slc17a9a-left TCTCCATTGGTCACGGAT
72 ZebrafishCommunity-slc17a9a-right TCTAGCCATGGTGACGGC
73 ZebrafishCommunity-slc17a9b-left TGCTCTACTGTGCGCGTG
73 ZebrafishCommunity-slc17a9b-right TCCGCCATGCTCACGGCA
74 ZebrafishCommunity-slit3-left TGGACAGGAATAACATCA
74 ZebrafishCommunity-slit3-right TTGAGGCCAGAGAAGTCC
75 ZebrafishCommunity-slmapb-left TGGAGGCCAGTGAGTCTG
75 ZebrafishCommunity-slmapb-right TGCAGTTCCTGGTTCAGC
76 ZebrafishCommunity-smyd1a-left TGGAGGTGTTCGGGGCCG
76 ZebrafishCommunity-smyd1a-right TCTCTGGTACCCCGCAGA
77 ZebrafishCommunity-snai1b-left TGAAGTTTCGAGGGGATA
77 ZebrafishCommunity-snai1b-right TCTTGACAAGAAATGAGC
78 ZebrafishCommunity-spermassociatedantigen7-left TTCTGGGCTCGATCTTGA
78 ZebrafishCommunity-spermassociatedantigen7-right TGATCGTGAACGGTTGGA
79 ZebrafishCommunity-sqstm1-left TGTCGATGACAGTGAAAG
79 ZebrafishCommunity-sqstm1-right TCCTTGTTGCAGTCCTCC
80 ZebrafishCommunity-srf-left TGGACGCTCTGGTAATGG
80 ZebrafishCommunity-srf-right TGTGCTGCCCACCTGTAG
81 ZebrafishCommunity-Stat3-left TGCAGCAGTTGGAGACGC
81 ZebrafishCommunity-Stat3-right TCGCTGTACAGGTGATAC
82 ZebrafishCommunity-stxbp1a-left TCAGCTTGCCCTGACCCC
82 ZebrafishCommunity-stxbp1a-right TGGAGGCACGGCTCTTCA
83 ZebrafishCommunity-Sun1-left TGGATTTTTCCCGTCTGC
83 ZebrafishCommunity-Sun1-right TTATCCGGAGTACAGTGA
84 ZebrafishCommunity-swap70b-left TAAGGGACGAGCTTCTC
84 ZebrafishCommunity-swap70b-right TCCAGGGCTGTGAAGG
85 ZebrafishCommunity-SYNE2-left TATGGGATGAGCTGGACC
85 ZebrafishCommunity-SYNE2-right TCGCCCTCTAACTCAGCT
86 ZebrafishCommunity-tbx4-left TGCTTCAGGAGAAGGCCT
86 ZebrafishCommunity-tbx4-right TGGGCAACGGTCATTCCT
87 ZebrafishCommunity-tetmethylcytosinedioxygenase1(previous-left
TCAACTCGCGCATCCACA
87 ZebrafishCommunity-tetmethylcytosinedioxygenase1(previous-right
TCATGGAAGCCTTCACCC
88 ZebrafishCommunity-tetmethylcytosinedioxygenase3-left
TTATTAGGCACTTTTTTG
88 ZebrafishCommunity-tetmethylcytosinedioxygenase3-right
TGTGGACAGGAAAGTGCA
89 ZebrafishCommunity-
tetmethylcytosinedioxygenase3(previoussi:ch211-220a11.1)-left
TCAAATCCTGAATCTCAA
89 ZebrafishCommunity-
tetmethylcytosinedioxygenase3(previoussi:ch211-220a11.1)-right
TTGTGCTGGCATTTTGAC
90 ZebrafishCommunity-TIMM22-left TTGTTCTGGGAGGTGCT
90 ZebrafishCommunity-TIMM22-right TTTGCATCAATGCCGG
91 ZebrafishCommunity-tppp3-left TGAGGGTTCGGGTGAAAT
91 ZebrafishCommunity-tppp3-right TTTACGAAATGCCATCTC
92 ZebrafishCommunity-trappc11-left TCCTATGAGTGGTACATT
92 ZebrafishCommunity-trappc11-right TGTTCATCCAGCCCGTCT
93 ZebrafishCommunity-ucma-left TGCTGATCGCGCTGACTG
93 ZebrafishCommunity-ucma-right TTTTCCATCTTTAACA
94 ZebrafishCommunity-vclbAL772263.1-left TGGCCCAGCAGATCTCCC
94 ZebrafishCommunity-vclbAL772263.1-right TCCACCTCTCCCTCCTCG
95 ZebrafishCommunity-ybx1-left TGAGCAGCGAGGCCGAGA
95 ZebrafishCommunity-ybx1-right TCCGCGTCGGCGGCAGGC
96 ZebrafishCommunity-yjefn3-left TTGAGACGGAGCTCCTGA
96 ZebrafishCommunity-yjefn3-right TCAATCAGCTGCTGCTGG
zfinadmn
Mar 4, 2013, 1:52:29 PM3/4/13
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