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Trizol extraction - zebrafish embryos
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Kim
Nov 4, 2009, 1:08:53 PM11/4/09
to bionet-organi...@moderators.isc.org
Has anyone encountered low 260/230 readings (<0.2) using the Trizol
RNA extraction. We're getting okay yields and great purity using 5
embryos (24hpf-72hpf)/sample, but are having problems with what we
assume is contamination.
RNA extraction. We're getting okay yields and great purity using 5
embryos (24hpf-72hpf)/sample, but are having problems with what we
assume is contamination.
We have already tried a number of things: bought all our chemicals
new, repeat chloroform extraction 2 times, do EtOH cleaning step 3
times. We homogenize manually with pestles.
Thanks for any advice.
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