WG: lactophenol

[Wilch, Antonia]

Dear Sir or Madam,

I am looking for Mr. Peter Werner who had a correspondence with David Brayford from IMI in the 1990ies. The content of this correspondence is very interesting for me, but I have some further questions. The e-mail address I found is not valid anymore, so I hope to get in contact with you in this way. I would be glad if somebody from your working group can answer my question from my e-mail below.

Yours sincerely,
Antonia Wilch
--
Georg-August-University Goettingen
Department of Crop Sciences
Division of Plant Pathology and Plant Protection
Grisebachstr. 6, D-37077, Goettingen

Von: Wilch, Antonia
Gesendet: Donnerstag, 25. Februar 2016 13:31
An: 'boe...@u.wahington.edu'
Betreff: lactophenol

Dear Mr. Werner,
I am PhD-student in the Division of Plant Pathology and Plant Protection at the University of Göttingen, Germany. I am writing you as a consequence of a quite curious discovery which is an e-mail from 1995 from you and whose content might help me with some further explanation from your side. I would be glad if my query results in clarity.
My project is aimed to find quantitative disease resistance mechanisms in oilseed rape against the harmful fungus Sclerotinia sclerotiorum. Since I am also performing studies on the microscope (CLSM), I am using an acid fuchsine staining method (see page 264 in attached paper from Eynck et al., 2007). The stain is solved in lactophenol. Preferring to find a less hazardous but strong staining, by searching our laboratory server by chance I found a correspondence between you and David Brayford from IMI (see attachment "lactophenol"). In your e-mail you explain that for the lactophenol blue staining with aniline blue you replace lactophenol by lactoglycerol. I hope I did not bore you with my long explanation, because now it is time for my question: do you have any knowledge or idea if for acid fuchsine staining I can replace lactophenol by lactoglycerol with the same success just as you did it? Or does it not fit with my stain? Unfortunately, we have no "staining professional" in our team; that is why I am referring to your 20 year old e-mail.
Thank you very much for your understanding.

Hoping for a positive answer,
With kind regards,
Antonia Wilch



--
Georg-August-Universität Göttingen
DNPW/APP
Grisebachstr. 6
37077 Göttingen

0551/39-12309