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green fluorescent protein

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ran...@ids.net

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Jul 18, 1995, 3:00:00 AM7/18/95
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In article <3ugp5e$i...@dartvax.dartmouth.edu>, Jennife...@dartmouth.edu (Susan K. Crosthwaite) writes:
> Has anyone expressed green fluorescent protein in Neurospora? If so,
> I'd be interested to hear what promoter(s) you used and whether or not
> the protein is stable and easy to detect. I'm thinking about hooking up
> GFP to the qa-2 and ccg-2 promoters and would just like to get a feel
> for whether it's going to be sucessfully expressed. Please Email me if
> you have any information Susan.K.C...@Dartmouth.EDU I will post
> a summary of the information I collect on the net. Thanks in advance,

> Sue Crosthwaite
Have you considered Pseudomonas contamination???? RRandolph


Susan K. Crosthwaite

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Jul 18, 1995, 3:00:00 AM7/18/95
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Jerome Rigot

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Jul 25, 1995, 3:00:00 AM7/25/95
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In article <3ugp5e$i...@dartvax.dartmouth.edu>,

Hi Sue,

I am also looking for a promoterto express green fluorescent protein in another filamentous fungus, Trichoderma harzianum. Can you send me the info you get? You may also contact Dr. Brett Tyler at UC DAvis, he has a weak to medium expression Neurospora promoter. His e-mail is: bmt...@ucdavis.edu
I, myself, am looking for a strong promoter.
--
Jerome Rigot
Dept. Environmental Toxicology
University of California
Davis, CA 95616
(916)752-5896
e-mail: jfr...@ucdavis.edu

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