Phage Display

[Simon Cross]

1) Does anyone know the best newsgroup for phage display?

2) I am using the Griffin1 library of phage displayed scFv's and can
never seem to get past the soluble fragment stage. I get very few
positives after 4 rounds of screening the phage, and I get reproducible
results of the clonal phage specifically binding my hapten molecule
shown by ELISA. However, when I express the soluble antibody from these
positives I never get the scFv binding in the same way (although it's
definitely expressed ok, shown by dot blot). I have heard of problems
with naive libraries and am aware that the scFv may not fold in the same

way when fused to the g3 coat protein, but there are papers out there
saying that this technique works! I would appreciate any suggestions,

Simon
pc...@nottingham.ac.uk

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[Mike Clark]

In article <39D1E671...@nottingham.ac.uk>, Simon Cross

Simon,

I can't answer your specific questions but I suggest that you try the
bionet.molecules.repertoires newsgroup (I have cross posted this reply).

One of the problems with published scientific techniques is that whilst
reports of success are readily published, reports of failure are not!

Mike Clark, <URL:http://www.path.cam.ac.uk/~mrc7/>
--
o/ \\ // || ,_ o M.R. Clark, PhD. Division of Immunology
<\__,\\ // __o || / /\, Cambridge University, Dept. Pathology
"> || _`\<,_ // \\ \> | Tennis Court Rd., Cambridge CB2 1QP
` || (_)/ (_) // \\ \_ Tel.+44 1223 333705 Fax.+44 1223 333875

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