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ER chaperones involved in peptide binding
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Miss M.S. Teng
Oct 27, 2000, 4:34:44 PM10/27/00
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I am studying peptide binding ER chaperones. As part of a crude assay, I
need a negative control for my experiment that involves any commercially available protein
that doesn't bind peptide. Are there any suggestions? Also, I understand
that certain ER chaperones (Example: ERp72) bind peptides to a lesser
extent than the more common ones like PDI.
To enable easy detection of its peptide binding activity, we could cross
link its antiserum to a solid phase matrix. I'm wondering if there are
other more efficient methods.
need a negative control for my experiment that involves any commercially available protein
that doesn't bind peptide. Are there any suggestions? Also, I understand
that certain ER chaperones (Example: ERp72) bind peptides to a lesser
extent than the more common ones like PDI.
To enable easy detection of its peptide binding activity, we could cross
link its antiserum to a solid phase matrix. I'm wondering if there are
other more efficient methods.
Thanks
Michelle Teng
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