Surfacelabelling of (Bacterial) proteins

[Peter Ashton]

Hello

I can't offer any suggestions, but out of interest, what is wrong with
biotinylation?

Pete

Per Mygind <pe...@biobase.dk> wrote in message news:7kncf4$5...@net.bio.net...
> Hello
>
> I'm just wondering wether any of you are aware of any methods for
> detection of surfaceexposed proteins in bacteria, apart from:
>
> Biotinylation
> Limited protease digestion
> Antigendetection by an antibody (EM, IMF)
> Surface-iodination
>
> Any other suggestions as to how to detect the proteins present on the
> surface of a given microorganism
>
> --
> Regards
>
> Per Mygind
>
> ************************************************************************
> If you are are not part of the solution, you are part of the precipitate
> ************************************************************************
>
> Per Mygind, Cand.scient
>
> Department of Medical Microbiology and Immunology
> The Bartholin Building, University of Aarhus, Denmark
> phone : 89 42 17 47, fax : 86 19 61 28
>
> ************************************************************************
> It's hard work and great art to make life not so serious
> ************************************************************************
> Life is what happens to you while you're busy making other plans
> (John Lennon)
>
> All you touch and all you see is all your life will ever be
> (Roger Waters)
>
>
>

[immunechem]

Internal biotinylated proteins (eg: carboxylase) may be
mis-interpreteated as surface protein.

I wonder if the epitope-labelling a good appraoch? Such as
anti-8-amino-7methyl isocoumarin (also fluorescent)?

In article <7lejm4$j...@net.bio.net>, pd...@york.ac.uk says...