lipid ox reply from BA Freeman

[Bruce Freeman]

Re your question on the freerad.net, I would recommend that for more
insightful
detection of lipid oxidation, that you initially measure TBA positive
material (Etsuo Niki
has good refs on this) and then, better, detect lipid hydroperoxides by HPLC
w/ UV
detection or by rxn w/ leukomethylene blue. Unless you do HPLC on TBA-lipid
ox
products to identify and quantify (w/ stds) the TBA-MDA adduct, the TBA rxn
will detect
a wide variety of biomolecules, many not even lipid-derived!

See: Rubbo, H. Parthasarathy, S., Kalyanaraman, B., Barnes, S., Kirk, M. and
Freeman, B.A. (1995) Arch. Biochem. Biophys. 324, 15-25 for use and the
original ref
of the LMB technique, that I learned of from Sam Parthasarathy, a wise guru
in the
measurement of oxidized lipid products. LOOH (and LOONO) analysis by HPLC is
detailed in a paper just being published from O'Donnell, VB and other members
of my
group in Biochemistry. This approach is not new, and has been utilized by
many in the
past for LOOH and LOH species.

Bruce Freeman