MDA e conjugated dienes
Monica Colitti
I would like to detect MDA and conjugated dienes in mammary homogenate
tissues. Have you some suggestions? Thanks. Monica
Monica Colitti
Dept. Animal Production Sciences
via delle Scienze, 208
33100 UDINE ITALY
Kevin Bown
> Date: 4 Dec 1997 07:43:24 -0800
> From: Monica Colitti <Monica....@Dspa.Uniud.It>
> Reply-to: Monica Colitti <Monica....@Dspa.Uniud.It>
> To: "bionet.molecules.free-radicals mail newsgroup"
<bione...@dl.ac.uk>
> Subject: MDA e conjugated dienes
> I would like to detect MDA and conjugated dienes in mammary homogenate
> tissues. Have you some suggestions? Thanks. Monica
>
Dear Monica
MDA can be detected colorimetrically (1), fluorescently (2) or by HPLC
(3) following adduct formation with thiobarbituric acid.
Conjugated dienes are measurable by ultraviolet spectroscopy (4) and lipid
hydroperoxide levels can be used as a correlative index to cojugated
dienes, when detected by HPLC (5). Most of the techniques are fairly
straightforward, hope this helps.
1) Buege, J.A and Aust, S.D (1978) Methods Enzymol. 52C, 302-310
2)Yagi, K (1984) Methods Enzymol. 105, 328-331
3) Bird R.P and Draper H.H (1984) Methods Enzymol. 105, 299-305
4) Recknagel, R.O and Glende, E.A (1984) Methods Enzymol. 105. 331-337
5) Slater, T.F and Sawyer, B.C (1971) Biochem. J 123 805-814
good luck .................. kevin bown
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Kevin Bown,
Centre for Applied Microbiology & Research,
Porton,
Wilts, SP4 OJG. UK.
Tel: +44(0)1980 612666
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e-mail: kevin...@camr.org.uk or kevi...@hotmail.com
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