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Mating efficiency for pretransformed library screening

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Junjie Shao

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Apr 1, 2013, 11:02:48 PM4/1/13
to ye...@magpie.bio.indiana.edu

Hi zhenming:

 

I find the “Mating efficiency for pretransformed library screening” in www.bio.net.

Now I have the same problem.

I do the yeast 2-hybrid screen by mating system from clontech. The efficiency is very low. I use the Y2H gold and Y187. Do you have any advice to me? Thank you.

 

Best

junjie

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