NovoZym 234

[Irene Jensen]

Does anyone know a good replacement for Novozyme 234 ( which is no
longer available). I need it in order to determine whether or not my
null mutants have an altered cell wall, that is I would like to do an
alpha-glucanse sensitivity assay. Sigma sells a product called Lysing
Enzymes but they do not test for alpha-glucanase activity. I have tried
to reproduce the published alpha-glucanase assay procedure with lysing
enymes but have been unable to do so. Any suggestions would be greatly
appreciated.
Irene Jensen
i-je...@uiuc.edu

[Bruce L. Miller]

We've purchased Novozyme 234 as recently as August 13 from:

Interspex Products
1155 Chess Dr. Ste. 114
Foster City, CA 94404
(415) 570-4337

10g Novo 234 for $245

--
Bruce L. Miller
Dept. of Microbiology, Molecular Biology & Biochemistry
University of Idaho
Moscow, ID 83843
bmi...@uidaho.edu

[Dr. Colin Rasmussen]

In article <bmiller-2009...@129.101.151.179>, bmi...@uidaho.edu
(Bruce L. Miller) wrote:

> In article <3242D9...@uiuc.edu>, i-je...@uiuc.edu wrote:
>
> > Does anyone know a good replacement for Novozyme 234 ( which is no
> > longer available). I need it in order to determine whether or not my
> > null mutants have an altered cell wall, that is I would like to do an
> > alpha-glucanse sensitivity assay. Sigma sells a product called Lysing
> > Enzymes but they do not test for alpha-glucanase activity. I have tried
> > to reproduce the published alpha-glucanase assay procedure with lysing
> > enymes but have been unable to do so. Any suggestions would be greatly
> > appreciated.
> > Irene Jensen
> > i-je...@uiuc.edu
>
> We've purchased Novozyme 234 as recently as August 13 from:
>
> Interspex Products
> 1155 Chess Dr. Ste. 114
> Foster City, CA 94404
> (415) 570-4337

We buy it from them and it is very good.

Colin Rasmussen

[Mark Prescott]

We use Zymolyase from the Seikagaku Corporation, Japan. Lyticase is
another option. We have not tried this one but it is available from
Boehringer.

Mark