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Green-ish yeast culture??
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Fan, Yao
Nov 7, 2013, 5:07:09 PM11/7/13
to ye...@magpie.bio.indiana.edu
Hi all,
I've been growing Saccharomyces cerevisiae (strain BJ2168) in shake flask for protein expression. After 72 hours' culture time I harvested the cells, but found a layer of suspicious-looking greenish goo on the edge of the pellet. After I resuspended the pellet in lysis buffer, I noticed that the the suspension also showed a trace of greenish color. I don't know if it is normal or is due to some bacterial contamination--I did have pen-strep added into the culture, so I'm really frustrated about the unknown green stuff. Anyone have had similar experience? Thanks!
Yao
I've been growing Saccharomyces cerevisiae (strain BJ2168) in shake flask for protein expression. After 72 hours' culture time I harvested the cells, but found a layer of suspicious-looking greenish goo on the edge of the pellet. After I resuspended the pellet in lysis buffer, I noticed that the the suspension also showed a trace of greenish color. I don't know if it is normal or is due to some bacterial contamination--I did have pen-strep added into the culture, so I'm really frustrated about the unknown green stuff. Anyone have had similar experience? Thanks!
Yao
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