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Bacterial promoters
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Jude_Pr...@med.uni-heidelberg.de
Aug 4, 2002, 12:27:37 PM8/4/02
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Dear List,
Whilst I have a bit of experience with parasite
promoters, I admit to being
a bit of an idiot as far as bacterial promoters are
concerned. My problem: I
would like to put a selectable marker under control
of 2 promoters, one of
parasite origin, and one bacterial. My parasite
promoter is not well
characterised, and therefore 1kb long. All bacterial
promoters that I have
looked at seem however to have a few ATGs scattered
within them, which I
think will cause a problem!! Is it feasible for me
to locate the bacterial
promoter upstream to the parasite one, adding
however the shine-dalgano
sequence just before the intended ATG? Can a
promoter work over such a great
distance? If not, then is it possible for me to
place a really minimal
promoter (-35, -10, SD ) of my own design downstream
of the parasitic one ,
making sure that no ATGs get in the way. I only need
enough expression to
enable cloning in e.coli.
Thanks in advance
Jude Przyborski
Parasitology
University of Heidelberg
Germany
ju...@web.de
---
Whilst I have a bit of experience with parasite
promoters, I admit to being
a bit of an idiot as far as bacterial promoters are
concerned. My problem: I
would like to put a selectable marker under control
of 2 promoters, one of
parasite origin, and one bacterial. My parasite
promoter is not well
characterised, and therefore 1kb long. All bacterial
promoters that I have
looked at seem however to have a few ATGs scattered
within them, which I
think will cause a problem!! Is it feasible for me
to locate the bacterial
promoter upstream to the parasite one, adding
however the shine-dalgano
sequence just before the intended ATG? Can a
promoter work over such a great
distance? If not, then is it possible for me to
place a really minimal
promoter (-35, -10, SD ) of my own design downstream
of the parasitic one ,
making sure that no ATGs get in the way. I only need
enough expression to
enable cloning in e.coli.
Thanks in advance
Jude Przyborski
Parasitology
University of Heidelberg
Germany
ju...@web.de
---
=====
Moderated
bionet.genome.gene-structure
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