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Recommendation for Fermenter for Pichia
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ani...@gmail.com
Jun 21, 2012, 12:47:23 PM6/21/12
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Dear all,
I am looking a buy a Fermenter for large scale (max. 4-5 l.) culture of Pichia to high cell densities. There are two models I am looking at
1) the Bioflo310 from New Brunswick
2) labfors 5 from ATR
Of course, I need all the usual stuff- temeprature control (through a water-jacketed system), O2 conentration control. pH control, feed lines for MeOH etc. Does anyone have any recommendation to make, from either of these or any other one ? Will be very helpful.
Best,
AB
I am looking a buy a Fermenter for large scale (max. 4-5 l.) culture of Pichia to high cell densities. There are two models I am looking at
1) the Bioflo310 from New Brunswick
2) labfors 5 from ATR
Of course, I need all the usual stuff- temeprature control (through a water-jacketed system), O2 conentration control. pH control, feed lines for MeOH etc. Does anyone have any recommendation to make, from either of these or any other one ? Will be very helpful.
Best,
AB
Biotecnologia - German
Jun 22, 2012, 10:28:57 AM6/22/12
to ani...@gmail.com, prot...@magpie.bio.indiana.edu
You could try Bioengeneering.
But I recommend a Sterilization in place Biorreactor. Like Bioflow 415. Is a hard task autoclave a 5 L jar in a laboratory autoclave (vertical). The problem will be the price.
The water jacket is another question, sometimes are difficult the temperature active the medium in sterilization process.
Do you consider to buy a Methanol analyzer for in process control?
Good look,
German Wassermann
-----Mensagem original-----
De: proteins...@oat.bio.indiana.edu [mailto:proteins...@oat.bio.indiana.edu] Em nome de ani...@gmail.com
Enviada em: quinta-feira, 21 de junho de 2012 13:47
Para: prot...@magpie.bio.indiana.edu
Assunto: [Protein-analysis] Recommendation for Fermenter for Pichia
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Proteins mailing list
Prot...@net.bio.net
http://www.bio.net/biomail/listinfo/proteins
But I recommend a Sterilization in place Biorreactor. Like Bioflow 415. Is a hard task autoclave a 5 L jar in a laboratory autoclave (vertical). The problem will be the price.
The water jacket is another question, sometimes are difficult the temperature active the medium in sterilization process.
Do you consider to buy a Methanol analyzer for in process control?
Good look,
German Wassermann
-----Mensagem original-----
De: proteins...@oat.bio.indiana.edu [mailto:proteins...@oat.bio.indiana.edu] Em nome de ani...@gmail.com
Enviada em: quinta-feira, 21 de junho de 2012 13:47
Para: prot...@magpie.bio.indiana.edu
Assunto: [Protein-analysis] Recommendation for Fermenter for Pichia
Proteins mailing list
Prot...@net.bio.net
http://www.bio.net/biomail/listinfo/proteins
Bohgosity BumaskiL
Sep 19, 2012, 5:47:56 PM9/19/12
to
On 2012-06-21 10:47 AM, ani...@gmail.com wrote about a fermenter
with O2 control.
Maybe Pichia like KNO3. There duzn't seem to be much that duz, and
what duz grows very slowly. I am guessing that the process of
biologically degrading nitrate requires rezistanse to chlorate or
chlorite. I might never know. I dumped my culture, so I don't know
if it wuz Pichia.
with O2 control.
Maybe Pichia like KNO3. There duzn't seem to be much that duz, and
what duz grows very slowly. I am guessing that the process of
biologically degrading nitrate requires rezistanse to chlorate or
chlorite. I might never know. I dumped my culture, so I don't know
if it wuz Pichia.
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