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Green Fluorescent Proteins
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Mahima Laroyia
Feb 23, 2011, 6:57:43 AM2/23/11
to fluo...@magpie.bio.indiana.edu
HI ,
I was reviewing the site and got to know some interesting facts about green fluorescent proteins but need to explore more. Can one answer my following questions ?
1. In what all case using green fluorescent protein to study gene expression fails ?
2. What are the developments that are still in progress to make GFP or its derivative better but are not published yet ?
Waiting for your replies
MAHIMA
Dr Mephesto
Jun 17, 2014, 3:04:23 PM6/17/14
to
1) I don't understand the question. Could you be more specific by 'fail'?
2) Topics for inprovement including GFP derivatives and widening to flourescent proteins in general include: Increasing brighness, lowering bleaching, increasing the palette range, making more long Stoke shift variants, making useful near IR fluorescent proteins, decreasing pH effect, making activatible or color shifting proteins, and making 2-photon or second harmonic generation variants. Thats without looking at all the fields of biosensors based on FP's.
-dave
2) Topics for inprovement including GFP derivatives and widening to flourescent proteins in general include: Increasing brighness, lowering bleaching, increasing the palette range, making more long Stoke shift variants, making useful near IR fluorescent proteins, decreasing pH effect, making activatible or color shifting proteins, and making 2-photon or second harmonic generation variants. Thats without looking at all the fields of biosensors based on FP's.
-dave
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