protein spectra and use in monitoring bacteria

[McDonald, David Joseph]

Hello all,

I'm currently working on a method to determine contributions of different fluorescent proteins from a total observed spectrum given off by a biofilm and, therefore, determine the growth of specific bacteria. I was wondering if anyone had knowledge of certain proteins fluorescing well with certain bacteria as opposed to others. I was also hoping someone would be able to provide me with the data or direct me to a journal or website that contains the data for full emission spectra for a variety of fluorescent proteins instead of just their maximum emission wavelength (which is what most websites list).

Specifically I'm searching for the emission spectra data for these proteins: mPlum, E2-Crimson, mRaspberry, HeRed1, mCherry, mStrawberry, AsRed2, DsRed-Monomer, DsRed2, tdTomato, DsRed-Express, DsRed-Express2, mOrange, mBanana, ZsYellow1, ZsGreen1, AcGFP1, Dendra2, Timer, AmCyan1, EYFP, ECFP. The spectra for EGFP was relatively easy to find.

I really appreciate all the help anyone can provide.

Sincerely,

David McDonald

[Dr Mephesto]

I have a lot of these proteins, but I have I haven't expressed and measured most of them all. You can find a lot though at: http://home.earthlink.net/~pubspectra/

There is a excel table with many proteins there. Too bad you can't get a grant for measuring them all, but TBH, I just don't have the time to analyse them all, as I just use the best and brightest.
-Dave