help! non-radioactive Southern

[Zhengyang Wu]

Hi! I have been working with P32- Southern, and now I want to begin with
non-radioactive labelling. There are many providers of non-radioactive-
labelling kits, but I don't know which are reliable.

I bought "ECL random prime labelling and detection system" from Amersham,
but later I found that this kit does not work with Southern of genomic
DNA. I feel me badly cheated.

There are other alternatives. Almost every bigger companies have own
non-radioactive labelling kits. Can someone who have experience in this
give me some suggestion which one I should buy for genomic DNA Southern?
Is there any other way to label DNA-probe without using harmful P32 for
this application?

Thanks in advance!

Zhengyang Wu, Ph.D student
Dept. Medical Microbiology
Linkoping University
S-581 85, Linkoping
Sweden
e-mail: zyw...@lysator.liu.se

Can anyone tell me how the world will look like after 5 million years?

[Guilherme Oliveira]

I have been using lately the S&S Rad-Free kit, and it has been working
well. It labels the nucleic acid (RNA, DNA, oligo) with psoralen/biotin.
Let me know if you need more details.
Good luck,
Gui.

In article <404n1d$c...@newsy.ifm.liu.se>, zyw...@lysator.liu.se (Zhengyang Wu) says:
>

>There are other alternatives. Almost every bigger companies have own
>non-radioactive labelling kits. Can someone who have experience in this
>give me some suggestion which one I should buy for genomic DNA Southern?
>Is there any other way to label DNA-probe without using harmful P32 for
>this application?
>

>e-mail: zyw...@lysator.liu.se

>
>Can anyone tell me how the world will look like after 5 million years?

______________________________________________
Guilherme Oliveira mpp...@vetmed.tamu.edu
Texas A&M University oliv...@tamu.edu
College of Vet. Med. phone (409) 862-4010
Dept. of Pathobiology fax (409) 862-2344
______________________________________________

[Tracey Weiler]

I have used the Boehringer Mannheim Genius system with random priming and
got quite nice clean film with intense signals. Works well with genomic
DNA and will detect single copy sequence.

Trace
-----------------------------------------------------
Tracey Weiler
Department of Biochemistry and Molecular Biology
University of Manitoba
770 Bannatyne Ave.
Winnipeg, MB
R3J 3S6 (204) 789 - 3231
FAX: (204) 783 - 0864
----------------------------------------------------