Ethidium Bromide -Vapors!!!
Antonieta Herrera
lot of steam!! I would like to know how toxic is the emanated
steam. The oven is outside of the lab air extractor.Is that correct?
I am still ok, thanks
DiogenesQV
Leman
Herrera") wrote:
> I always melt agorose/TA+Ethidium in a microwave oven. Uhmm a
>lot of steam!! I would like to know how toxic is the emanated
>steam. The oven is outside of the lab air extractor.Is that correct?
I don't know about the EtBr vapor, but I don't see any good reason to
melt agarose with EtBr added. First of all, you greatly increase the
chances of spilling it all over the place in case of overboiling.
Second, you destroy part of EtBr by boiling. And third -- what stops
you from doing it the right way and adding EtBr after agarose cools
down to 55C? This way you can melt your agarose in your kitchen
microwave and not worry about any EtBr toxicity.
Leman
D.Schnapp
Sure, I'll always do it in the correct way if possible, but I have been
known to forget to pour the gel and having to re-melt the agarose :} ...
Also, some people wonder about re-using agarose trimmings.
Cheers,
Denni
****************************************************************************
Denni Schnapp
Dept. of Paediatric Gastroenterology
St. Bartholomew Hospital
Dominion House Phone:(0)171/6018469
59 Bartholomew Close Fax: (0)171/6005901
London EC1A 7BE http://www.st-and.ac.uk/~www_sa/personal/ds4/
****************************************************************************
---
V.S. Gautam
bromide. As far as I am aware, the "bromide" part of any chemical is very
toxic and dangerous if inhaled. It might not have immediate effect, but
yes, on longterm they sure do have.
Sorry, I may sound too open-mouthed, but I am surprised at the kind of
questions posed on this forum for toxicity/dangerousness about chemicals
which are known to be toxic to us (humans) by people working in biological
sciences. I presume we all have studied at sometime of our BS and MS that
most of these chemicals are dangerous, and if people lookup the labels on
the bottles it will also say what damage it will cause, etc etc.....so why
not just take precautions instead of asking questions on this forum??
Take care and best of luck for all the exps with such chemicals.....
---------------------------------------------------------------------------
Gautam V Sondarva,
Department of Microbiology and Cell Biology,
Indian Institute of Science,
Bangalore 560 012,
India.
Phone: +91 80 309 2703
Fax: +91-80-3444697, 3341683, 3342085
E Mail: gau...@mcbl.iisc.ernet.in
-----------------------------------------------------
Judge a person by his questions, not his answers.
-----------------------------------------------------
---
Rene Nederhand
I don't know about the EtBr vapor, but I don't see any good reason to
[cut]
-- what stops
you from doing it the right way and adding EtBr after agarose cools
down to 55C? [cut...]
The reason is (at least for me) that I use my gels for more than one time.
After use I melt then and pour again. This works at least three times and
saves agarose. I think EtBr will be degraded rapidly at high temperature, so
I don't see a health problem.
Rene
**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you have received this email in error please notify
the sender.
This footnote also confirms that this email message has been swept by
MIMEsweeper and Dr. Solomon Toolkit for the presence of computer viruses.
**********************************************************************
---
Dr Dave Parcej
chemical on your shelf it is classified as potentially deleterious to your
health (you should see the paper that comes when we order Tween 80!). For
some reason, people are particularly worried about ethidium bromide, yet
it classified only as "harmful". Acyrlamide and PMSF are much more toxic
but there doesn't seem to be so much anxiety regarding the use of these
compounds! Just be careful with any lab chemical.
Cheers
Dave
In article <Pine.LNX.4.10.99092...@mcbl.iisc.ernet.in>,
> > > I don't know about the EtBr vapor, but I don't see any good reason to
> > > melt agarose with EtBr added. First of all, you greatly increase the
> > > chances of spilling it all over the place in case of overboiling.
> > > Second, you destroy part of EtBr by boiling. And third -- what stops
> > > you from doing it the right way and adding EtBr after agarose cools
Peter
<Dun...@genesys.demon.co.uk> wrote:
>
> I like what it says in the Merck index.
>
> Bitter tasting dark red crystals ...
>
> I wonder what happened to the taster.
LOL
Peter
Dr. Duncan Clark
V.S. Gautam <gau...@mcbl.iisc.ernet.in> writes
>Sorry, I may sound too open-mouthed, but I am surprised at the kind of
>questions posed on this forum for toxicity/dangerousness about chemicals
>which are known to be toxic to us (humans) by people working in biological
>sciences.
I like what it says in the Merck index.
Bitter tasting dark red crystals ...
I wonder what happened to the taster.
It was of course also used therapeutically in animals as an
antiprotozoal.
Duncan
--
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk
Caroline Szymeczek-Seay
let me preface this by saying that i almost always add EtBr after
microwaving the agarose. but i have worked with people who like to make
up 500 ml flasks of agarose with EtBr, which they then nuke every time
they need a gel. The compromise solution was that they, instead of
microwaving the solution with the EtBr in it, instead heated it on low
on a heat plate in the fume hood. Works fine, as long as you don't
crank it on high.
clss
Antonieta Herrera wrote:
>
> I always melt agorose/TA+Ethidium in a microwave oven. Uhmm a
> lot of steam!! I would like to know how toxic is the emanated
> steam. The oven is outside of the lab air extractor.Is that correct?
>
Peter
par...@biophys.mpg.de (Dr Dave Parcej) wrote:
> Acyrlamide and PMSF are much more toxic
> but there doesn't seem to be so much anxiety regarding the use of these
> compounds! Just be careful with any lab chemical.
When we went thru Lab safety class(we have to do this every year), the guy
giving the lecture just said "It is best if you assume everything is
tetra-methyl-death and treat it as such"
regards
Peter
Nick Theodorakis
> look at almost *ANY*
> chemical on your shelf it is classified as potentially
> deleterious to your
> health (you should see the paper that comes when we
> order Tween 80!).
You should see the warning label I saw on a bottle of
caffeine!
Nick
(BTW, isn't Tween the same thing as polysorbate?)
* Sent from RemarQ http://www.remarq.com The Internet's Discussion Network *
The fastest and easiest way to search and participate in Usenet - Free!
Leman
<c...@med.unc.edu> wrote:
>hi,
>let me preface this by saying that i almost always add EtBr after
>microwaving the agarose. but i have worked with people who like to make
>up 500 ml flasks of agarose with EtBr, which they then nuke every time
>they need a gel. The compromise solution was that they, instead of
>microwaving the solution with the EtBr in it, instead heated it on low
>on a heat plate in the fume hood. Works fine, as long as you don't
>crank it on high.
Even better yet: make up a big flask of it and keep it in a 65C
incubator. This way you just don't have to reheat it.
Leman
Bill_A_N...@ms.bd.com
Content-type: text/plain; charset=us-ascii
Content-Disposition: inline
Bill A Nussbaumer@BDX
09/24/99 02:29 PM
Often when I make up larger amounts of gel than I need, rather than nuking it
again I'll just go ahead and pour it and store it in the fridge under buffer.
Even if it's not exactly what I need in my next experiment I'm always certain to
use it in the near future.
Just a thought,
Bill
hi,
let me preface this by saying that i almost always add EtBr after
microwaving the agarose. but i have worked with people who like to make
up 500 ml flasks of agarose with EtBr, which they then nuke every time
they need a gel. The compromise solution was that they, instead of
microwaving the solution with the EtBr in it, instead heated it on low
on a heat plate in the fume hood. Works fine, as long as you don't
crank it on high.
clss
Antonieta Herrera wrote:
>
> I always melt agorose/TA+Ethidium in a microwave oven. Uhmm a
> lot of steam!! I would like to know how toxic is the emanated
> steam. The oven is outside of the lab air extractor.Is that correct?
>
> I am still ok, thanks
> DiogenesQV
--0__=sxKKRtR9XxzgK5bBlmOmBYBeYoRbdbQhJdBBnhUhWBvPplvITvR8EP2u
Content-type: application/octet-stream;
name="css.vcf"
Content-Disposition: attachment; filename="css.vcf"
Content-transfer-encoding: base64
YmVnaW46dmNhcmQgDQpuOlNlYXk7Q2Fyb2xpbmUNCngtbW96aWxsYS1odG1sOkZBTFNFDQpvcmc6
UGVkaWF0cmljcw0KYWRyOjs7Ozs7Ow0KdmVyc2lvbjoyLjENCmVtYWlsO2ludGVybmV0OmNzc0Bt
ZWQudW5jLmVkdQ0KZm46Q2Fyb2xpbmUgU2VheQ0KZW5kOnZjYXJkDQoNCg==
--0__=sxKKRtR9XxzgK5bBlmOmBYBeYoRbdbQhJdBBnhUhWBvPplvITvR8EP2u--
Roland Hübner
& many others recycling gels by boiling...
Hi,
I have been told that EtBr is _VERY_ heat stable (above 1000oC)...
Roland
Brendan Scott
overzealous technical officers tried making mass amounts of agarose for
gels for a while, but found that after several rounds of
remelting/reboiling in the microwave, the agarose would go brown and be
unuseable.
Incidentally, the technical officer in question is now heavily pregnant,
and I will be happy to provide a list of birth defects to methods-reagents
after the delivery.
Cheers.
++++++++++++++++++++++++++++++++++++++++++
Brendan Scott
Human Genetics Group
John Curtin School of Medical Research
Australian National University
Canberra ACT 0200
AUSTRALIA
Ph: (026) 249 0417/3725
Fax (026) 249 4712
0418 871 514
++++++++++++++++++++++++++++++++++++++++++
Mark Dowton
s314...@STUDENT.ANU.EDU.AU (Brendan Scott) wrote:
I absolutely agree with Bill and Peter's response to this, and I think
both were being too nice. This is completely tasteless, and could only
come from someone who has never had children. Are you observing your
colleague (I wouldn't use 'friend') as a mutagenicity trial? You would
never work in my lab.