Fisher Taq shipped on dry ice
Ken Soderstrom
I just got 100U of Taq from Fisher that arrived frozen solid on dry ice.
I'm worried about the effect of freezing on its activity. I hate to waste
my time proving to Fisher that this is a bad idea. Their tech support
claims that this is thier standard shipping method which doesn't seem right.
Does anyone out there know if its OK to freeze Taq.
Thanks much,
Ken Soderstrom, Ph.D.
http://www.psy.fsu.edu/~soderstrom/
Todd Richmond
<soder...@psy.fsu.edu> wrote:
> I just got 100U of Taq from Fisher that arrived frozen solid on dry ice.
>
> I'm worried about the effect of freezing on its activity. I hate to
> waste
> my time proving to Fisher that this is a bad idea. Their tech support
> claims that this is thier standard shipping method which doesn't seem
> right.
>
> Does anyone out there know if its OK to freeze Taq.
That's the standard way we store Taq for long-term storage. It's a bad
idea to freeze Taq polymerase during the purification process, but once
it's in 50% glycerol (storage buffer) it's fine for several years.
Todd
--
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Dr. Todd Richmond
Carnegie Insitution, Dept of Plant Biology
260 Panama Street
Stanford, CA 94305
Dr. Duncan Clark
Florida State University wrote
>I just got 100U of Taq from Fisher that arrived frozen solid on dry ice.
>
>I'm worried about the effect of freezing on its activity. I hate to waste
>my time proving to Fisher that this is a bad idea. Their tech support
>claims that this is thier standard shipping method which doesn't seem right.
>
>Does anyone out there know if its OK to freeze Taq.
Do not panic it will be fine frozen.
My concern would not be the freezing but the dry ice, as in CO2 vapour!
We have experienced problems in the past with shipping Taq enzyme
containing a red dye, which also happens to be a nice pH indicator. It
has been seen more than once that on some tubes the CO2 vapour gets
passed a well sealed screw capped microfuge tube with 'O' ring
(Sarstedt) such that the dye goes from deep red/purple to orange or even
yellow due to formation of carbonic acid. The standard Taq storage
buffer is only 10 or 15mM Tris and it doesn't take much to shift the pH
from 8 to say 7. Not enough to kill the enzyme short term but long term
may have an effect. Since then, we use heat sealed bags for shipping in
dry ice.
Duncan
--
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk
Warren Gallin
Warren Gallin
R. Jayakumar
presence of glycerol. Probably you may check up with the company on this
matter rather than on the dry ice matter, since any way, that is how mostly
all companies send their enzymes.
jayakumar
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Dr. Duncan Clark
Jayakumar at School of Biotechnology wrote
>we also get our taq in dry ice, and we have never seen it in freeze, due to
>presence of glycerol.
But even 50% glycerol Taq storage buffer should freeze in dry ice. It
does in a -70C freezer.
R. Jayakumar
enzymes in freeze packs. You are right, since dry ice has a temperatures
of -78C or 109C, it should freeze. But does this freezing really affect the
activity.
jayakumar
> But even 50% glycerol Taq storage buffer should freeze in dry ice. It
> does in a -70C freezer.
>
> Duncan
> --
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....
>
> Duncan Clark
> GeneSys Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382
> http://www.dnamp.com
> http://www.genesys.demon.co.uk
>
>
>
---
Dr. Duncan Clark
Jayakumar at School of Biotechnology wrote
>activity.
Not noticed any with Taq over a number of years!
Dr. Duncan Clark
Duncan Clark at DNAmp Ltd. wrote
>In article <005b01c094f3$a99ee320$8c01...@tn.nic.in>, the eminent R.
>Jayakumar at School of Biotechnology wrote
>>But does this freezing really affect the
>>activity.
>
>Not noticed any with Taq over a number of years!
Insert ' problem '