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Does anybody know how to extract protein from agarose?
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Jing Wang
Dec 30, 2009, 1:45:41 PM12/30/09
to met...@magpie.bio.indiana.edu
HI~
Does anybody know how to extract protein from agarose? I want to use the extracted proteins to do further iTRAQ experiment.
Thank you so much! Happy new year:)
Best!
Jing Wang
Dept. of Biomedical & Chemical Engineering
Syracuse University
jwa...@syr.edu
Nick Theodorakis
Dec 30, 2009, 5:13:56 PM12/30/09
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On Dec 30, 1:45 pm, Jing Wang <jwan...@syr.edu> wrote:
> HI~
>
> Does anybody know how to extract protein from agarose? I want to use the extracted proteins to do further iTRAQ experiment.
>
> HI~
>
> Does anybody know how to extract protein from agarose? I want to use the extracted proteins to do further iTRAQ experiment.
>
You can use agarase (commercially available) or electrophoresis into a
dialysis membrane or to derivatized paper (DEAE, e.g.). I would guess
even the old "freeze and squeeze" might work.
Nick
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Nick Theodorakis
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