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Centricon concentrator re-use

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xianghuai Lu

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Mar 14, 1997, 3:00:00 AM3/14/97
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Hi,
Has anyone out there re-used disposable concentrators? If so, how
long do they keep, how are they cleaned, etc. I have heard that they
can be cleaned with EtOH. Any comments or suggestions would be
appreciated, as these are too expensive to throw away one per sample.
Thanks in advance,
Adam Keeton
Molecular and Cellular Pathology
University of Alabama at Birmingham

Matthew L. Brown, Ph.D

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Mar 14, 1997, 3:00:00 AM3/14/97
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Adam,

We reuse concentrators but only for the same protein. (i.e. don't
cross-contaminate) I believe we got a protocol from the centricon manual
or the Amicon catalog. At any rate we soak the filter units in 70 %
ethanol and never let them dry. Just before reusing them we rinse them
and spin with the same buffer in which our protein is solubilized. (i.e.
PBS) Then we load on the new protein and spin. Hope this helps. It seems
to work fine for us.

Matthew L. Brown, Ph.D.
Dept. of Medicine
Univ. of Alabama at Birmingham

P.S. I know you from Steve's lab I think...

Anne E. Hall

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Mar 16, 1997, 3:00:00 AM3/16/97
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Hi-
I have re-used the centricon 30's. I don't know just how long you can
reuse them, but I have successfully re-used them up to four times. In
between uses I store them with 70% EtOH and wrap them with parafilm and
keep them in the fridge.
A. Hall
University of Wisconsin-Madison

--
Anne E. Hall
U.W. Madison
aeh...@students.wisc.edu

Frances E. Chen

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Mar 18, 1997, 3:00:00 AM3/18/97
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In article <aehall-1603...@arabidopsis.botany.wisc.edu> aeh...@students.wisc.edu (Anne E. Hall) writes:
>
>Hi-
>I have re-used the centricon 30's. I don't know just how long you can
>reuse them, but I have successfully re-used them up to four times. In
>between uses I store them with 70% EtOH and wrap them with parafilm and
>keep them in the fridge.

That's interesting. We have tried to store centripreps in water:EtOH and
then found that they no longer worked properly. I figure the filter
material is the same in the centricons and centripreps. We store ours in
water with a little sodium azide - seems to work fine.

Frances Chen
Univ.CA at San Diego
fc...@jeeves.ucsd.edu

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