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Gycogen co-precipitation with DNA?

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Joel Bader

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Nov 20, 1996, 3:00:00 AM11/20/96
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Can anyone help me with the use of glycogen as a means of visualizing
DNA during precipitation? I would like to co-precipatate DNA with
gycogen during ethanol precipitation. I've heard it can be used
successfully for that application. I would like any references and
any instructions you can provide. Thanks in advance!
Joel


Kathie Grovit-Ferbas

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Nov 25, 1996, 3:00:00 AM11/25/96
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I use 40 micrograms per extraction and have had great success. I order
the glycogen (oyster) from Fluka catalog number 50573.

Paul N Hengen

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Nov 25, 1996, 3:00:00 AM11/25/96
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Joel Bader (bad...@aub.mindspring.com) wrote:

@article{Hengen1996Junetibs,
author = "P. N. Hengen",
title = "Methods and reagents - Carriers for
precipitating nucleic acids",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "6",
pages = "224-225",
month = "jun",
year = "1996"}

@article{Helms1985,
author = "C. Helms
and M. Y. Graham
and J. E. Dutchik
and M. V. Olson",
title = "A new method for purifying lambda
{DNA} from phage lysates",
journal = "DNA",
volume = "4",
number = "1",
pages = "39-49",
comment = "mussel glycogen as carrier",
year = "1985"}

@article{Tracy1981,
author = "S. Tracy",
title = "Improved rapid methodology for the
isolation of nucleic acids from agarose gels",
journal = "Preparative Biochem.",
volume = "11",
number = "3",
pages = "251-268",
comment = "oyster glycogen used as carrier",
year = "1981"}

--
*******************************************************************************
* Paul N. Hengen, Ph.D. /--------------------------/*
* National Cancer Institute |Internet: p...@ncifcrf.gov |*
* Laboratory of Mathematical Biology | Phone: (301) 846-5581 |*
* Frederick Cancer Research and Development Center| FAX: (301) 846-5598 |*
* Frederick, Maryland 21702-1201 USA /--------------------------/*
* - - - Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - - *
* - TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - - *
* - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/ *
*******************************************************************************

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