Stability of IPTG?
WS
I wonder how stable IPTG is in aqueous solutions. It seems to dissolve
very well to give a 1M stock, but does it make sense to do it that way,
i.e how long is IPTG stable in water? At room temp?
I know it's soluble in DMF and DMSO and said to be quite stable therein
when stored at -20. As I will use it to induce protein synthesis in E.
coli in quite large cultures, I would like to avoid organic solvents
this time. And DMSO also is converted to a quite stenchy smell when the
bugs are allowed to play with it for some time. So I'd prefer to go
with water.
Best regards,
Wo
Christian Praetorius
>I wonder how stable IPTG is in aqueous solutions. It seems to dissolve
>very well to give a 1M stock, but does it make sense to do it that way,
>i.e how long is IPTG stable in water? At room temp?
Why do you want to keep it at room temperature? We usually made a
larger batch in water (eg 10ml), made 1ml aliquots and stored them at
-20. We never observed any problems.
Christian
--
X-no-Sig: yes
WS
Christian Praetorius wrote:
>
> Why do you want to keep it at room temperature?
... happened by accident, stood there for some time, and the stock
which I relied on has not been refilled. Shit happens. Had to use the
water solution for one megaprep and had in my mind (lab myth?) that
IPTG would degrade when in contact with water for prolonged time.
> We usually made a
> larger batch in water (eg 10ml), made 1ml aliquots and stored them at
> -20. We never observed any problems.
thats good news!
Many Thanks!
Wolfgang
>
> Christian