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RNase inactivation by UV

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Scott Craighead

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Jul 4, 2000, 3:00:00 AM7/4/00
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Hi,

Has anyone used a UVItec CL 508 UV crosslinker to inactivate
RNase
on miscellaneous labware? Do you find this to be effective?

Thanks for any help,

Scott.

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Dr. Hiranya S. Roychowdhury

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Jul 5, 2000, 3:00:00 AM7/5/00
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Never knew that RNases could be inactivated by UV? What is the chemistry
behind it?

Dr. Hiranya Sankar Roychowdhury
Dept. of Molecular Biology
PO Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroy...@nmsu.edu


---

David F. Spencer

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Jul 25, 2000, 3:00:00 AM7/25/00
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In article <003dc1d6...@usw-ex0102-013.remarq.com>, Scott
Craighead <ascraighe...@yahoo.com.invalid> wrote:

> Hi,
>
> Has anyone used a UVItec CL 508 UV crosslinker to inactivate
> RNase
> on miscellaneous labware? Do you find this to be effective?
>
> Thanks for any help,
>
> Scott.

I can't imagine how that could be at all effective. For flasks,
beakers, etc. it's the inside surface that you want RNase free but
glass (and many/most plastics) very effectively blocks UV shorter than
about 300 nm, which means that the inside surfaces will receive little
or no useful UV exposure. It is possible that in a very air-tight
container with lots of 254 nm UV there will be enough ozone generated
by the UV to knock out RNases, etc.

Dave

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