Yellow EDTA ph8, 0.5M

Sharon Rho

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Jul 21, 2008, 2:55:31 PM7/21/08

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Has anyone had problems with EDTA turning a clear yellow solution? I used the same protocol that others in the lab used: 186.1 g of EDTA, add 500 ml of distilled water, then add NaOH pellets until a pH of 8 is reached...but it still turns out yellow. Does anyone know how to solve the problem? Thanks.

chovek69

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Jul 23, 2008, 3:59:33 AM7/23/08

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On Jul 21, 9:55 pm, Sharon Rho <janshar...@yahoo.com> wrote:
> Has anyone had problems with EDTA turning a clear yellow solution? I used the same protocol that others in the lab used: 186.1 g of EDTA, add 500 ml of distilled water, then add NaOH pellets until a pH of 8 is reached...but it still turns out yellow. Does anyone know how to solve the problem? Thanks.
>

I suppose the EDTA powder is old...Is it clearly white or is
yellowish ?

Han

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Jul 23, 2008, 6:59:48 AM7/23/08

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chovek69 <iva...@gmail.com> wrote in
news:dfae68a1-9aa7-4648...@b1g2000hsg.googlegroups.com:

Or there is tea in the system, or the NaOH pellets were really old ...

--
Best regards
Han
email address is invalid

Dr. Hiranya S. Roychowdhury

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Jul 22, 2008, 10:54:36 PM7/22/08

to jansh...@yahoo.com, met...@magpie.bio.indiana.edu

It is common, and varies from batch to batch. Usually it is not a problem.
If using for sensitive rxns, simply filter it through a 0.2micron filter.

> Has anyone had problems with EDTA turning a clear yellow solution? I used
> the same protocol that others in the lab used: 186.1 g of EDTA, add 500 ml
> of distilled water, then add NaOH pellets until a pH of 8 is reached...but
> it still turns out yellow. Does anyone know how to solve the problem?
> Thanks.
>
>
>
>

> _______________________________________________
> Methods mailing list
> Met...@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>

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Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003

Jayakumar, R

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Jul 23, 2008, 9:22:42 AM7/23/08

to jansh...@yahoo.com, met...@magpie.bio.indiana.edu

Which company did you buy it from??
Jay

-----Original Message-----
From: methods...@oat.bio.indiana.edu [mailto:methods...@oat.bio.indiana.edu] On Behalf Of Sharon Rho
Sent: Monday, July 21, 2008 2:56 PM
To: met...@magpie.bio.indiana.edu
Subject: Yellow EDTA ph8, 0.5M

Has anyone had problems with EDTA turning a clear yellow solution? I used the same protocol that others in the lab used: 186.1 g of EDTA, add 500 ml of distilled water, then add NaOH pellets until a pH of 8 is reached...but it still turns out yellow. Does anyone know how to solve the problem? Thanks.

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