In article <
mailman.112.151033...@net.bio.net>,
bmac...@unc.edu says...
>
> Hello,
>
> We are looking into vital stains for bacterial vesicle and vacuole membranes, especially any that might be sensitive to lipid composition. Any information along those lines would be welcome, actually. But the particular question - can anyone point us to detailed characterization of MDY-64? It is used as a yeast vacuolar stain, but the papers I?ve been able to find don?t say much about its structure or why it might show this specificity, and the manufacturer's (Thermo
Fisher, of course) technical support couldn?t find anything in their records.
>
Have you already checked the molecular probes catalogue (available from
ThermoFisher, who aquired MP some years back)?
At least in plants and yeast, the vacuole is quite acidic and
accumulates basic dyes that are membrane-permeble only in the uncharged
state (e.g., acridine orange).