bisacrylamide not dissolving

[Siddhartha Parida]

I am trying to make a 29:1Acrylamide: bisacrylamide solution in
deionized water , but the bisacrylamide is not desolving ? any
suggestion on why is it so ?

[WS]

bis acrylamide is not very well soluble in water: about 20g/l

Wo

[Pow Joshi]

On 3/2/07, Siddhartha Parida <sidh...@gmail.com> wrote:
> I am trying to make a 29:1Acrylamide: bisacrylamide solution in
> deionized water , but the bisacrylamide is not desolving ? any
> suggestion on why is it so ?

with that particular concentration it should ...... you could try
29.2: 0.8, not that it makes too much of a difference ..... also, you
should add the water in small amounts, since the volume expansion is
quite a bit, and you will have exceeded the final volume ...... hope
that is helpful

pow
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[Dr. Hiranya S. Roychowdhury]

bad/old batch. Get rid of it and buy a fresh bottle.
Quoting Siddhartha Parida <sidh...@gmail.com>:

> I am trying to make a 29:1Acrylamide: bisacrylamide solution in
> deionized water , but the bisacrylamide is not desolving ? any
> suggestion on why is it so ?
>

> _______________________________________________
> Methods mailing list
> Met...@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>

--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Dept. of Health and Public Services, Rm# 191T,
Dona Ana Community College of New Mexico State University
MSC 3DA,
Las Cruces, NM 88003

[peter]

How on earth you know that this is the bis-acrylamide that does not
dissolve? Anyway, try the microwave and heat it up.
Peter