Google Groups no longer supports new Usenet posts or subscriptions. Historical content remains viewable.
Dismiss
LiCl precipitation?
3 views
Skip to first unread message
Ed Siefker
Mar 7, 2013, 4:29:10 PM3/7/13
to met...@magpie.bio.indiana.edu
I did an alkaline lysis miniprep and ended up with gobs and gobs of RNA.
I added an equal volume of 4M LiCl, chilled at -20C for 40 minutes, and
spun at 16K Gs for 20 minutes. I don't see a pellet. What am I doing wrong?
I added an equal volume of 4M LiCl, chilled at -20C for 40 minutes, and
spun at 16K Gs for 20 minutes. I don't see a pellet. What am I doing wrong?
0 new messages