How to prepare 1M H2O2 using 30% hydrogen peroxide?
WANG XF
I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2 using
30% hydrogen peroxide?
Thank you all!
Lao WANG
Bean Long
Aawara Chowdhury
WANG XF <wxf...@gmail.com> wrote:
> I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2 using
> 30% hydrogen peroxide?
The molecular weight of H2O2 is 34 daltons (or close enough to 34).
1M H2O2 = 34 g/L
30% H2O2 = 300 g/L
Do the math, and dilute it in ddH2O.
AC
--
Email: echo 36434455860060025978157675027927670979097959886449930P | dc
Dr. Hiranya S. Roychowdhury
30% H2O2 is 300g/Liter
1M H2O2 is 34g/Liter (approx)
Will that suffice?
(This is what most of us are amazed about! Apoptosis is a good thing ---
thank God!)
> Hello!
>
> I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2
> using
> 30% hydrogen peroxide?
>
> Thank you all!
>
> Lao WANG
> _______________________________________________
> Methods mailing list
> Met...@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003
Han
news:qHDpj.14164$M24....@newsfe17.lga:
> In <mailman.479.12020...@net.bio.net>,
> WANG XF <wxf...@gmail.com> wrote:
>
>> I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2
>> using 30% hydrogen peroxide?
>
> The molecular weight of H2O2 is 34 daltons (or close enough to 34).
> 1M H2O2 = 34 g/L
> 30% H2O2 = 300 g/L
>
> Do the math, and dilute it in ddH2O.
>
> AC
Don't mouth-pipet the 30%.
3 % is OK, but don't overdo it - it tastes bad.
<grin>
--
Best regards
Han
email address is invalid
Pow Joshi
>
>
>
> 30% H2O2 is 300g/Liter
>
> 1M H2O2 is 34g/Liter (approx)
>
> Will that suffice?
>
> (This is what most of us are amazed about! Apoptosis is a good thing ---
> thank God!)
LOL! ... sorry I could'nt help but notice your last remark about
apoptosis....
Pow
> Hello!
> >
> > I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2
> > using
> > 30% hydrogen peroxide?
> >
WANG XF
**
*30% H2O2 is 300g/L? or 300L/L or 300g/g?*
**
*Some of my colleagues have different suggestions.*
**
*Thank you!*
**
*Lao WANG*
------------------------------
>
> Message: 2
> Date: Mon, 4 Feb 2008 11:29:15 +0800
> From: "WANG XF" <wxf...@gmail.com>
> Subject: How to prepare 1M H2O2 using 30% hydrogen peroxide?
> To: met...@magpie.bio.indiana.edu
> Message-ID:
> <404daa2f0802031929ne6...@mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello!
>
> I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2
> using
> 30% hydrogen peroxide?
>
> Thank you all!
>
> Lao WANG
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 04 Feb 2008 15:49:22 +1100
> From: Bean Long <ben....@yourfinger.anu.edu.au>
> Subject: Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?
> To: met...@net.bio.net
> Message-ID: <47a69952$1...@clarion.carno.net.au>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> WANG XF wrote:
> > Hello!
> >
> > I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2
> using
> > 30% hydrogen peroxide?
> >
> > Thank you all!
> >
> > Lao WANG
>
> Here's a handy little link:
>
> http://www.trimen.pl/witek/calculators/stezenia.html
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 04 Feb 2008 12:38:46 GMT
> From: Aawara Chowdhury <aaw...@pontiff-playground.org>
> Subject: Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?
> To: met...@net.bio.net
> Message-ID: <qHDpj.14164$M24....@newsfe17.lga>
>
> In <mailman.479.12020...@net.bio.net>,
> WANG XF <wxf...@gmail.com> wrote:
>
> > I want to use H2O2 as an inducer of apoptosis. How to prepare 1M H2O2
> using
> > 30% hydrogen peroxide?
>
> The molecular weight of H2O2 is 34 daltons (or close enough to 34).
> 1M H2O2 = 34 g/L
> 30% H2O2 = 300 g/L
>
> Do the math, and dilute it in ddH2O.
>
> AC
> --
> Email: echo 36434455860060025978157675027927670979097959886449930P | dc
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Met...@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 33, Issue 3
> **************************************
>
WS
that's the real issue. All your assumptions may be true.
IMHO is is *absolutely* necessary to specify m/v (mass/volume), m/m or
v/v. It even makes a difference if you (in this case rather
hypothetically as solid H2O2 does not exist [at least for a long
time]) mix 300 ml of H2O2 and 700ml of water, put 300 ml H2O2 in a
beaker and add water up to 1liter or vice versa. Does not make always
a real difference in the outcome of an experiment, but when you need
to troubleshoot a protocol or reproduce an experiment, this kind of
things will drive you nuts. There is nothing like a 'common sense' on
how to make procentual solutions. If possible, always state molar
contents, therefore.
All the best,
Wo
On 5 Feb., 15:45, "WANG XF" <wxfh...@gmail.com> wrote:
> *Thanks for your help!*
> **
> *30% H2O2 is 300g/L? or 300L/L or 300g/g?*
> **
> *Some of my colleagues have different suggestions.*
> **
> *Thank you!*
> **
> *Lao WANG*
> > **************************************
Pow Joshi
>
> In article <
> 4f8737e1-bbe4-4542...@e6g2000prf.googlegroups.com>, WS <
> novalid...@nurfuerspam.de> wrote:
> >Dear Lao,
> >
> >that's the real issue. All your assumptions may be true.
> >
> >IMHO is is *absolutely* necessary to specify m/v (mass/volume), m/m or
> >v/v. It even makes a difference if you (in this case rather
> >hypothetically as solid H2O2 does not exist [at least for a long
> >time]) mix 300 ml of H2O2 and 700ml of water, put 300 ml H2O2 in a
> >beaker and add water up to 1liter or vice versa.
>
> the case, for example, with concentrated HCl (~ 37% ~ 12M)
> or concentrated H3PO4 (85% = 15.2M).
>
> MSDS for 30% H2O2 says "balance water", suggesting
> that indeed we are talking about 300 g + 700 g water.
> In which case precise molarity calculattion become
> troublesome even knowing density of 100% H2O2
> because water solutions can change volume
> appreciably (e.g. 500 ml ethanol + 500 ml H2O
> will result in less than 1000 ml solution).
>
> Not that any of that matters in the experiment in
> question! Assuming no volume change and m/m
> for 30% and density off 199% of 1.4 g/ml from
> Wikipedia, we get 22-28% error depending on the
> direction. With the "1 M" being clearly an arbitrary
> round number, I wouln't worry about potential
> 25% error as long as the way the solution is prepared
> is clearly stated.
yes, that is true.... H2O2 would be w/w.... the MSDS generally does'nt give
the information, however, calling the company would give. Frankly, I did'nt
know that this is true for all industrial preparation..... Thank you Dima.
Pow
DK
Virash Gupta
all the best
On Feb 7, 2008 10:34 PM, <methods...@oat.bio.indiana.edu> wrote:
> Send Methods mailing list submissions to
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> Today's Topics:
>
> 1. R: buffer preparation (Prof. Piero Sestili)
> 2. Dulbecco`s PBS vs PBS pH7.4 (AS)
> 3. Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?
> (Pow Joshi)
> 4. RE:Induction of apoptosis (Ozan Aygun)
> 5. Re: Dulbecco`s PBS vs PBS pH7.4 (DK)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 6 Feb 2008 16:02:26 +0100
> From: "Prof. Piero Sestili" <piero....@uniurb.it>
> Subject: R: buffer preparation
> To: <bertr...@gmail.com>, <met...@magpie.bio.indiana.edu>
> Message-ID: <002501c868d1$4d9d7f00$7d0c...@iram.it>
> Content-Type: text/plain; charset="iso-8859-1"
>
> First prepare a 2 M solution by diluting 2.26 ml of your stock to 10 ml,
> then make serial 1:10 dilutions down to 2 mM
> or
> prepare a 200 mM solution first diluting 226 ul of your stock to 10 ml
> and then another 1:100 dilution to 2 mM. As you prefer....
>
>
> Piero
>
>
> Prof. Piero Sestili
> Istituto di Farmacologia e Farmacognosia e
> Centro di Ricerca sull'Attività Motoria
> Università degli Studi di Urbino "Carlo Bo"
> Via "I Maggetti" 26
> 61029 URBINO (PU)
> Tel. 0722 303414; 0722 305524
> Fax 0722 303401
>
>
> -----Messaggio originale-----
> Da: methods...@oat.bio.indiana.edu
> [mailto:methods...@oat.bio.indiana.edu] Per conto di
> bertr...@gmail.com
> Inviato: mercoledì 6 febbraio 2008 12.11
> A: met...@magpie.bio.indiana.edu
> Oggetto: buffer preparation
>
> Hello,
>
> How do i work this out?
> :
>
> I need 2mM H2O2 from a stock solution H2O2 of 30%, density 1.11 gr/ml,
> M.W. 34
>
>
> anybody?
> thanks upfront
>
> b
> _______________________________________________
> Methods mailing list
> Met...@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 6 Feb 2008 11:39:05 -0800 (PST)
> From: AS <Antonio...@gmail.com>
> Subject: Dulbecco`s PBS vs PBS pH7.4
> To: met...@net.bio.net
> Message-ID:
> <8dbed6dc-131a-4266...@e23g2000prf.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> What is the difference between Dulbecco`s PBS vs PBS pH7.4?
>
> Does it make a difference if I just use it to wash cells (293, MEFs
> etc) before trypsination or lysation?
>
> Thanks!
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 6 Feb 2008 13:09:45 -0500
> From: "Pow Joshi" <pow....@gmail.com>
> Subject: Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?
> To: DK <d...@no.email.thankstospam.net>
> Cc: met...@magpie.bio.indiana.edu
> Message-ID:
> <710764ea0802061009j765...@mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> ------------------------------
>
> Message: 4
> Date: Wed, 6 Feb 2008 13:45:51 -0800 (PST)
> From: Ozan Aygun <metuge...@yahoo.com>
> Subject: RE:Induction of apoptosis
> To: met...@magpie.bio.indiana.edu
> Message-ID: <16475.9...@web90402.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi,
>
> You may want to give try alpha-amanitin, which is a
> highly specific inhibitor of RNA polymerase II. It is
> known that exposure to this reagent causes apoptosis
> in human cells.
>
> Good luck,
>
> Ozan
>
>
>
> ____________________________________________________________________________________
> Looking for last minute shopping deals?
> Find them fast with Yahoo! Search.
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>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 06 Feb 2008 22:25:02 GMT
> From: d...@no.email.thankstospam.net (DK)
> Subject: Re: Dulbecco`s PBS vs PBS pH7.4
> To: met...@net.bio.net
> Message-ID: <1tqqj.50$iB4...@newsfe07.lga>
>
> In article <
> 8dbed6dc-131a-4266...@e23g2000prf.googlegroups.com>, AS <
> Antonio...@gmail.com> wrote:
> >What is the difference between Dulbecco`s PBS vs PBS pH7.4?
>
> Probably none. Gibco used to specify its Dulbecco's PBS
> PH as 7.0-7.5. "PBS" is a mess. It can refer to a number of
> similar solutions, each with and without Ca2+ and/or Mg2+.
>
> >Does it make a difference if I just use it to wash cells (293, MEFs
> >etc) before trypsination or lysation?
>
> In the range in question, pH won't make a difference but for
> trypsinization there might be a diffrence between PBS
> containing divalents or not. Don't think it will be significant
> if you use trypsin-EDTA.
>
> DK
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
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>
> End of Methods Digest, Vol 33, Issue 6
> **************************************
>
--
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210
thekin...@gmail.com
HYDROGEN PEROXIDE
c(H2O2) = 1 mol/L
=======================
To prepare 100 mL of a 1 mol/L solution of hydrogen peroxide we will need to dilute 5.68513 mL of 50 % H2O2 to a final volume of 100 mL with deionized (distilled) water.
SOURCE:
-------------
Name: Hydrogen peroxide
Formula: H2O2
Formula weight: 34.015 g/mol
CAS Number: 7722-84-1
NFPA: Health 3, Flammability 0, Instability 1, Special OX
CALCULATION:
-------------
The key concept is that the amount of solute in the desired solution must be equal to the amount of solute in the source solution. Remember, the concentration is the amount of a solute divided by the volume of the solution.
Before we make any calculations we have to make sure that we only use one system and one unit of measurement. DO NOT mix measurement systems and units.
Desired solution:
V0 = 100 mL * (1 L)/(1000 mL) = 0.1 L
c0 = 1 mol/L
Source solutions:
w1 = 50 % = 50 * 1/100 = 0.5
d1 = 1.19662 kg/L * (1 g/L)/(0.001 kg/L) = 1196.62 g/L
--------------------------------
Then, we determine the concentration of the source (stock) solution
c1 = d(H2O2) * w(H2O2) / M(H2O2)
c1 = 1196.62 g/L * 0.5 / 34.0147 g/mol
c1 = 17.5897 mol/L
Since the total amount of solute is the same before and after dilution, the volume of stock solution needed is
V1 = V0 * c0 / c1
V1 = 0.1 L * 1 mol/L / 17.5897 mol/L
V1 = 0.00568513 L
To convert the result into a desired unit we will use dimensional analysis again
V(50 % H2O2) = 0.00568513 L * (1000 mL)/(1 L) = 5.68513 mL
PROCEDURE:
-------------
First of all, fill the volumetric flask about halfway with deionized water to avoid violent reactions. NEVER add water to concentrated acid.
Choose a clean pipette of suitable size and transfer the liquid to the volumetric flask. When the whole solution has been drained, touch the tip of the pipette to the side of the volumetric flask to allow the last of the liquid to drain out. DO NOT blow out the remaining solution.
Allow the solution to reach room temperature because a volumetric flask is only accurate at the temperature at which it has been calibrated (usually 20 癈). Very carefully fill the flask to the mark on the neck of the flask, using a dropping pipette to add the last few milliliters of liquid. Mix your solution thoroughly, by inverting the flask and shaking. NEVER hold large volumetric flasks by the neck alone - provide support at the bottom.
Transfer the prepared solution to a clean, dry storage bottle and label it. NEVER store solutions in a volumetric flask.
SAFETY NOTES:
-------------
- When making chemical solutions, always use the appropriate safety equipment.
- As a general rule, always add the more concentrated solution to the less concentrated solution.
- All chemicals that you are unfamiliar with should be treated with extreme care and assumed to be highly flammable and toxic.