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CTAB method of DNA purification

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Eva Pietrzak

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Apr 16, 1996, 3:00:00 AM4/16/96
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Hello, netters

I have a questions regarding the CTAB method of purifying bacterial DNA I can not
answer with the few books close to my hand :

In Current protocols in Molecular Biology (Eds. Ausubel et al) DNA is extracted first
with chloroform/isoamyl, then with phenol/chloroform/isoamyl.
Is there a reason for this reversal of the normal order of extraction, or is it a mistake
in the protocol?


Thanks, Eva

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