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Dilute nitric acid for cleaning glassware and lab tools
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John Dudgeon
Oct 19, 2015, 2:23:21 PM10/19/15
to Met...@magpie.bio.indiana.edu
Does anyone have an opinion on the utility of dilute HNO3 (~2-3%) for
removing residual DNA and proteins from glassware and stainless steel lab
tools. Our water is very hard here (>35 grains hardness), so we use HNO3 to
periodically clean lab glassware from hard water deposits in large dunk
tanks.
Our experience in our tace metals and DNA lab suggest nitric acid is far
less damaging to polyethylene and polypropylene plastics, glassware with
painted volumetric markings and stainless steel tools than dilute NaClO
(chlorine bleach) solutions. We have used HNO3 for years to digest tissues
and metalloproteins prior to ICP-MS analysis where we are trying to release
incorporated trace metals, and I wonder if it might be equally as effective
as NaClO for removing DNA and RNA on labware used in forensic DNA and/or
protein applications. There are many papers that discuss the method of
action of HNO3 on organic molecules, but I haven't seen anybody
specifically referencing its effect on DNA/RNA.
Any opinions?
John
--
John Dudgeon, Ph.D.
Associate Professor, Department of Anthropology
Director, Center for Archaeology, Materials and Applied Spectroscopy (CAMAS)
------------------------------------------------------------------------------------------------
Idaho State University, 921 S. 8th Avenue, Stop 8005, Pocatello, ID
83209-8005
Phone: (208) 282-3862 - FAX: (208) 282-4944
http://anthropology.isu.edu/dudgeon.shtml
http://www.isu.edu/camas/
removing residual DNA and proteins from glassware and stainless steel lab
tools. Our water is very hard here (>35 grains hardness), so we use HNO3 to
periodically clean lab glassware from hard water deposits in large dunk
tanks.
Our experience in our tace metals and DNA lab suggest nitric acid is far
less damaging to polyethylene and polypropylene plastics, glassware with
painted volumetric markings and stainless steel tools than dilute NaClO
(chlorine bleach) solutions. We have used HNO3 for years to digest tissues
and metalloproteins prior to ICP-MS analysis where we are trying to release
incorporated trace metals, and I wonder if it might be equally as effective
as NaClO for removing DNA and RNA on labware used in forensic DNA and/or
protein applications. There are many papers that discuss the method of
action of HNO3 on organic molecules, but I haven't seen anybody
specifically referencing its effect on DNA/RNA.
Any opinions?
John
--
John Dudgeon, Ph.D.
Associate Professor, Department of Anthropology
Director, Center for Archaeology, Materials and Applied Spectroscopy (CAMAS)
------------------------------------------------------------------------------------------------
Idaho State University, 921 S. 8th Avenue, Stop 8005, Pocatello, ID
83209-8005
Phone: (208) 282-3862 - FAX: (208) 282-4944
http://anthropology.isu.edu/dudgeon.shtml
http://www.isu.edu/camas/
Dr S.A.D.P. Senadheera
Oct 21, 2015, 1:00:31 PM10/21/15
to John Dudgeon, Met...@magpie.bio.indiana.edu
one of the best options to remove traces of nucleic acids is to rinse the
glassware after thorough wash with 1M NaOH.
cheers!
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glassware after thorough wash with 1M NaOH.
cheers!
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> Met...@net.bio.net
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>
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