How do I apply the genome scale reconstruction for E. coli in my experiments

[Yoginee Budhkar]

Hi all,

I have after a lot of struggle managed to develop a strain of E. coli that
produces squalene. Now I want to find out genes that I can target to
increase the synthesis of squalene. I have read a lot of papers on
constrain based and dynamic models to find out these targets, but honestly
I am not trained in this area and do not understand much of it.

I came across few articles by B. O. Palsson and his group where they have
made genome-scale reconstruction for E. coli. Where do I get it? and how do
I use it for my system???

Any help is appreciated...

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Yoginee Budhkar
Contact: +91 9820870988