Re: Recycling Coomassie destain

[m.fari...@gmail.com]

You can do that by putting a filter paper on top of a funnel and put some activated carbon on that, then add destain and let it go through activated carbon and filter to the bottle under. This worked perfectly for us.



On Thursday, July 24, 1997 at 3:00:00 AM UTC-4, Wolfgang Schechinger wrote:
> Hi.
>
> I suggest distillation of the mix. But I can't warrant that, if you
> store the sol'n, that you also will get methyl acetate since MeOH and
> HOAc will react (in a chemical equilibrium) to the ester. I only can
> say that I used a premixed sol'n stored at RT for more than three
> weeks without problems.
>
> You alternatively could switch to a more water soluble dye a ponceau
> red, that is easily destained with a buffer containing gelatin or
> othe proteins.
>
> Wolfgang
>
> > Dear colleagues,
> >
> > In order to reduce the amount of chemical waste, our lab would like
> > to recycle the MeOH/HAc destain we use for Coomassie stained gels,
> > by removing the stain. Has anyone figured out a convenient way of
> > doing this? Is activated carbon the best thing to use, or will
> > filtration through a thick stack of paper do?
> >
> > Any suggestions are welcome,
> > Thanks,
> >
> > Marc Werten (m.w.t....@ato.dlo.nl)
> > Dept. Microbial Conversions
> > Agrotechnological Research Institute (ATO-DLO)
> > Bornsesteeg 59
> > 6708 PD Wageningen
> > The Netherlands
> >
> > FAX: +31.317.475347
> >
> >
> >
> -----
>
> Wolfgang Schechinger
> University of Tuebingen
> email: wgsc...@med.uni-tuebingen.de
>
>
> http://www.medizin.uni-tuebingen.de/~wgschech/research.htm