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non fluorecent way for nuclear staining

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merav karsenty

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Dec 27, 2009, 6:43:37 AM12/27/09
to met...@magpie.bio.indiana.edu
Hello,
Is there a non fluorecent way for nuclear staining?

thanks
Merav

Sébastien Vigneau

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Dec 27, 2009, 9:51:52 AM12/27/09
to merav karsenty, met...@magpie.bio.indiana.edu
Haematoxylin?

Sébastien Vigneau

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Nick Theodorakis

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Dec 28, 2009, 9:10:41 AM12/28/09
to
On Dec 27, 9:51 am, Sébastien Vigneau <sebastien.vign...@gmail.com>
wrote:
> Haematoxylin?

I was thinking of that, too.

Some clarification from the OP would help, too. Is it that you don't
have access to a fluorescent scope, or that you don't want the stain
to be fluorescent? Because if it is the latter, I believe that
hematoxylin is autofluorescent; or at least, some component of an H&E
stained slide is autofluorescent.

What is the nature of your sample? Live, fixed or frozen? Cells or
tissues? How are you visualizing it now?

Nick

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merav karsenty

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Dec 28, 2009, 1:40:32 PM12/28/09
to Nick Theodorakis, met...@magpie.bio.indiana.edu
I need the stain to be non fluorecent because I dont have acess to a
fluorecent microscop. I know Haematoxylin, but as I anderstand, using this
stain must involve fixation. I am working with living cells and I am trying
to find a way to stain them without fixation, unless there is no choice. Is
there a way that you know?

2009/12/28 Nick Theodorakis <nick.the...@gmail.com>

Sébastien Vigneau

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Dec 28, 2009, 2:38:02 PM12/28/09
to merav karsenty, Nick Theodorakis, met...@magpie.bio.indiana.edu
Maybe Nile Blue, or Neutral
Red<http://www.histosearch.com/histonet/Jul00A/Re.fastredneutralornuclea.html>
.

Sébastien Vigneau


On Mon, Dec 28, 2009 at 1:40 PM, merav karsenty <mer...@gmail.com> wrote:

> I need the stain to be non fluorecent because I dont have acess to a
> fluorecent microscop. I know Haematoxylin, but as I anderstand, using this
> stain must involve fixation. I am working with living cells and I am
> trying
> to find a way to stain them without fixation, unless there is no choice. Is
> there a way that you know?
>
> 2009/12/28 Nick Theodorakis <nick.the...@gmail.com>
>

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