About TE and Tris-HCl sterilize with autoclave

[fabio]

I want know if a cycle of 120°C for 20'-30' in autoclave can damage the
TE and TRis-HCl solutions.
Thanks in advance.

Fabio

[Tom Knight]

fabio <fa...@fabius.it> writes:
> I want know if a cycle of 120°C for 20'-30' in autoclave can damage
> the TE and TRis-HCl solutions.

No, but a typical autoclave might leave them less pure than you might like.
If I cared about that, I'd filter sterilize.

[Jose de las Heras]

"Tom Knight" <t...@csail.mit.edu> wrote in message
news:vuybreh...@soggy-fibers.csail.mit.edu...

Seconded.

Don't bother autoclaving eppendorfs and tips, they're cleaner straight from
the box!

Jose

[Dr. Hiranya S. Roychowdhury]

It is done all the time ... autoclaving, i mean. Neither tris, nor EDTA,
breaks down in the autoclave.

Quoting fabio <fa...@fabius.it>:

--
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
---

[Ned Mantei]

In article <vuybreh...@soggy-fibers.csail.mit.edu>,
Tom Knight <t...@csail.mit.edu> wrote:

Filter sterilizing will not inactivate, e.g., RNase.
But I agree with the later poster mentioning not autoclaving Eppendorf
tubes and pipet tips--they are "machine sterile" as they come out of
the form (like bacterial Petri dishes, which are not sterilized in an
extra step).

--
Ned Mantei
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland

[Kyle Legate]

Ned Mantei wrote:
> In article <vuybreh...@soggy-fibers.csail.mit.edu>,
> Tom Knight <t...@csail.mit.edu> wrote:
>
>> fabio <fa...@fabius.it> writes:
>>> I want know if a cycle of 120°C for 20'-30' in autoclave can damage
>>> the TE and TRis-HCl solutions.
>>
>> No, but a typical autoclave might leave them less pure than you
>> might like. If I cared about that, I'd filter sterilize.
>
> Filter sterilizing will not inactivate, e.g., RNase.
>

Neither will autoclaving. Baking is required to kill RNAse.

[fabio]

Anyway baking isn't possible for a buffer, autoclaving is good to
inactivate DNAse and DEPC used (if) to destroy RNAse, but DEPC isn't
good for
Tris solution.

[fabio]

thanks to all!

[Kyle Legate]

Correct. The best you can do is use DEPC-treated water to make tris buffer
using a bottle of tris dedicated to RNAse free work (no spatulas, no
replacing overweighed amounts).