Request for dam–/dcm– E. coli

[Bjorn]

Hi, I wonder if anyone would be willing to share an
dam–/dcm– negative E. coli like for example the NEB dam–/dcm–.

I have a plasmid I need to produce w/o methylation.

Thanks!
/Bjorn

[Björn Johansson]

Dear Takehide

Thank you for the tip, I could find dam dcm strains, but I am
really looking for one that is also endA so that plasmid quality and yield
does not suffer.

all the best,
bjorn
______O_________oO________oO______o_______oO________O_________oO________
Björn Johansson, Assistant Professor
Department of Biology
University of Minho
Campus de Gualtar
4710-057 Braga
PORTUGAL
https://metabolicengineeringgroupcbma.github.io | www.bio.uminho.pt
Office direct +351-253 601517 | mob. +351-967 147 704 | Dept secretary
+351-253 60 4310


On Thu, Dec 6, 2018 at 11:33 PM M. Take at Tsukuba Science City <
take_...@hotmail.com> wrote:

> Dear Bjorn ,
>
> Can you find such the strain in the National BioResource Project stock?
> https://shigen.nig.ac.jp/ecoli/strain/
>
> Takehide
> NBRP E.coli Strain - shigen.nig.ac.jp
> <https://shigen.nig.ac.jp/ecoli/strain/>
> "jw621-am"といった単語を二重引用符 (") で囲むと、完全一致検索を行うことができます。
> shigen.nig.ac.jp
>
> ------------------------------
> *差出人:* methods...@oat.bio.indiana.edu <
> methods...@oat.bio.indiana.edu> が Bjorn <bjor...@gmail.com> の代理で送信
> *送信日時:* 2018年12月7日 0:23
> *宛先:* met...@magpie.bio.indiana.edu
> *件名:* Request for dam–/dcm– E. coli

> _______________________________________________
> Methods mailing list
> Met...@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>